Evaluation of AgNOR counts in pre-cancerous and cancerous cervix
Journal Title: Medpulse International Journal of Gynaecology - Year 2017, Vol 3, Issue 3
Abstract
Background: The AgNOR technique which was used extensively in cytogenetics earlier has now gained importance as an indicator of cell proliferation. cytology may fail to identify low grade and high grade squamous intraepithelial lesions which would progress to invasive cancers. In such cases, silver-stained nucleolar organizing regions (AgNOR) can be used. The study was conducted to evaluate AgNOR counts in pre-cancerous and cancerous cervix. Material and Methods: A total of 50 carcinoma cervix patients were included during the two years period from 2004 to2006. 25 cases served as control. The cervical and vaginal smears collected from controls and from patients were subjected to AgNOR staining. AgNOR counting was carried out as proposed by Chiu et al. Results: Most patients presented initially at an advanced stage (>IIB) moderately differentiated squamous cell carcinoma was the commonest type observed. AgNOR technique provided an index of cell proliferation. AgNOR count/cell increased gradually from normal to SIL changes to invasive carcinomas. The AgNOR count was high in poorly differentiated carcinomas and low in well differentiated carcinomas. The observations revealed significant increase of AgNOR count in cancerous lesions than in pre-cancerous lesions. Conclusion: AgNOR count can be used to assess the cellular proliferation rate. This simple silver staining technique can be used as an adjunct to routine cytological and histopathologic examination for early diagnosis.
Authors and Affiliations
Saraswathi D, Sathiyabama D
Keywords
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