EVALUATION OF ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY OF ROOTS OF WALTHERIA INDICA
Journal Title: World Journal of Pharmaceutical Research - Year 2019, Vol 8, Issue 2
Abstract
Background and Objective: The aim of this study was the determine in vitro antibacterial activity of different extracts of Waltheria Indica roots against standard bacteria, determine the Minimum Inhibitory Concentrations (MICs), study the cytotoxic effect of active extracts against different cell line and identify the major chemical components of the plants extracts. Material and Method: The cup agar plate diffusion method was used to screen the antibacterial activity of plant extracts and antibiotic. The Minimum Inhibitory Concentrations (MICs) of the most active extracts were determined using the agar plate dilution method. Micro-culture-tetrazolium MTT-assay was utilized to evaluate the cytotoxicity of extracts. All data were expressed as mean ± standard Deviation mean. Analysis of variance was performed by ANOVA using the SPSS software. Significant differences between means were determined by Least Significant Difference (L.S.D) and student t-test. A significant difference was considered at the level of P <0.05. Results: The results indicated that most extracts exhibited inhibitory effect against stranded bacteria. The lowest minimum inhibitory concentration (MIC) values was <3.125 mg/ml. Ps.aeruginosa, E.coli, S.aureus and B.subtilis were sensitive to Amoklan at concentration 100μg/ml, while S.aureus was resistant. Most extracts inhibited organism more than drug used. Alkaloid, flavonoids, tannins, saponins, sterols and triterpenes were found in extracts. The results of Micro-culture-tetrazolium (MTT) assayrevealed that all extracts tested were non-toxic.
Authors and Affiliations
Dr. Rajaa H. Tabidi
Keywords
Related Articles
- EP ID EP667026
- DOI -
- Views 126
- Downloads 0
IMIPENEM RESISTANCE AMONG GRAM NEGATIVE BACILLI FROM GREAT KWA RIVER, NIGERIA
Emergence of antibiotics resistance bacteria is a persistent global problem affecting public health. The occurrence and widespread resistance to carbapenems among environmental bacterial isolates can constitute a major t...
DIABETIC RETINOPATHY AND ITS SYSTEMIC RISK FACTORS
Diabetes mellitus (DM), commonly referred to as diabetes, is a group of metabolic disorders with complications that include sight-threatening diabetic retinopathy, also known as diabetic eye disease. To implement an effe...
THE IMPACT OF WORKING MOTHER'S ON THE NUTRITIONAL STATUS OF CHILDREN AGED 4-24 MONTHS IN KHARTOUM STATE
Objective: To assess the impact of work of mothers on the nutritional status of children aged 4-24 months in Khartoum state. Methodology: The study sample were 100 working and 100 non-working mothers who have children ag...
STUDY OF KNOWLEDGE, ATTITUDE AND PRACTICE OF SAUDI WOMEN TOWARDS PHYSICAL ACTIVITY, 2017
Background: Physical activity is an effective component of healthand prevention of disease. Increasing the general health knowledge about physical activity could prevent obesity and chronic diseases. Objectives: Study th...
CLINICAL ASPECT OF TEMPORAL REGION WSR TO SRUNGATAKA MARMA AS DESCRIBED IN INDIAN SYSTEM OF MEDICINE
From the time immemorial, they were keen in unravelling the secrets of this most sophisticated and complicated machinery. Ayurveda with a broad approach had explained the human body considering its various aspects- metap...