EVALUATION OF ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY OF ROOTS OF WALTHERIA INDICA
Journal Title: World Journal of Pharmaceutical Research - Year 2019, Vol 8, Issue 2
Abstract
Background and Objective: The aim of this study was the determine in vitro antibacterial activity of different extracts of Waltheria Indica roots against standard bacteria, determine the Minimum Inhibitory Concentrations (MICs), study the cytotoxic effect of active extracts against different cell line and identify the major chemical components of the plants extracts. Material and Method: The cup agar plate diffusion method was used to screen the antibacterial activity of plant extracts and antibiotic. The Minimum Inhibitory Concentrations (MICs) of the most active extracts were determined using the agar plate dilution method. Micro-culture-tetrazolium MTT-assay was utilized to evaluate the cytotoxicity of extracts. All data were expressed as mean ± standard Deviation mean. Analysis of variance was performed by ANOVA using the SPSS software. Significant differences between means were determined by Least Significant Difference (L.S.D) and student t-test. A significant difference was considered at the level of P <0.05. Results: The results indicated that most extracts exhibited inhibitory effect against stranded bacteria. The lowest minimum inhibitory concentration (MIC) values was <3.125 mg/ml. Ps.aeruginosa, E.coli, S.aureus and B.subtilis were sensitive to Amoklan at concentration 100μg/ml, while S.aureus was resistant. Most extracts inhibited organism more than drug used. Alkaloid, flavonoids, tannins, saponins, sterols and triterpenes were found in extracts. The results of Micro-culture-tetrazolium (MTT) assayrevealed that all extracts tested were non-toxic.
Authors and Affiliations
Dr. Rajaa H. Tabidi
Keywords
Related Articles
- EP ID EP667026
- DOI -
- Views 128
- Downloads 0
A FORMULA FOR PREVENTING CARDIOVASCULAR DISEASE IN HEALTHY INDIVIDUALS AT RISK
Patients suffering from cardiovascular disease are usually treated with concomitant administration of cholesterol lowering drugs such as atorvastatin, aspirin and beta blockers such as propranolol HCl. Therefore, the obj...
KNOWLEDGE, ATTITUDE AND PRACTICE OF ROAD TRAFFIC ACCIDENTS AMONG CAR DRIVERS IN MAJMAA CITY, SAUDI ARABIA, 2017-2018
Background: Due to the fast pace and evolution of Al-Majmaa from being a small governorate to a city which in turn will increase RTAs as well as the increase of unaware population due to insufficient research and lack of...
FLUORIDE AND ITS INTERACTION WITH HUMAN LIFE - A REVIEW
The objective of this comprehensive review is to update the impact of Sodium Fluoride on human system and other life on the earth. Sodium Fluoride is a source of Fluoride ion used in miscellaneous applications includes h...
TRANSMISSION OF ZIKA VIRUS FROM INFECTED MOTHER TO FETUS
Zika virus (ZIKV) is a mosquito borne flavivirus that has become threatens to public health infecting both adults’ and new-borns. It was characterized by maculopapular rash, fever, non-purulent conjunctivitis and arthral...
KNOWLEDGE ATTITUDE AND PRACTICE REGARDING PARACETAMOL USE AMONG MEDICAL AND NON-MEDICAL STUDENTS OF THE UNIVERSITY OF KHARTOUM, SUDAN.
Background: Paractamol is the most commonly used analgesic worldwide. It is one of the over the counter (OTC) drugs and is an effective analgesic in treating mild to moderate pain and fever. Aim: The aim of the study is...