Evaluation of Antibacterial Efficacy of Digera Muricata Plant using Microbroth Dilution and Autobiography Assays
Journal Title: International Journal of Botany and Research (IJBR) - Year 2018, Vol 8, Issue 2
Abstract
INTRODUCTION Bacterial infections treatment always remained as a challenge due to development of resistance in bacterial species. OBJECTIVE Medicinal plants contain a wide range of diverse molecules that provide a platform to search novel anti-bacterial agents. MATERIAL & METHODS Digera Muricata plant material was sequentially extracted with five different solvents. Plant extracts were screened against gram +veand gram –ve bacteria for their antibacterial activity using micro-broth dilution and thin layer chromatography (TLC) based bioassay RESULTS Plant extracts of D. muricatas showed minimum inhibitory concentration (MIC) in a range of 0.156-5.0 mg/ml. A decreased MIC was observed along with the increased polarity of extraction solvents. TLC guided bio-assay of active plant extracts against different bacteria developed in different solvent systems. TLC of D. muricata chloroform extracts was were developed in T:Ea:M::7:2:1 solvent system. Bio-autography of D. muricata chloroform extract showed two zones of bacterial inhibition. CONCLUSION Plant extract exhibiting significant MIC does not necessarily shows the zone of bacterial inhibition in TLC based bioassays. Moreover, different extracts showed selective activity toward selective bacterial strains that can be used to identify the specific pathways in the bacteria or against specific types of infections.
Authors and Affiliations
ASHISH MALIK, SUMAN LATA, PREETI JAIN
Keywords
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- EP ID EP343816
- DOI 10.24247/ijbrapr20182
- Views 93
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