Evaluation of Anticancer Potentials of Bee Free Venom a nd Chitosan Nano - Conjugated One: In Vitro Study
Journal Title: International Journal of Scientific Research and Management - Year 2017, Vol 5, Issue 4
Abstract
Limited efficacy of the current first - line treatment for cancer cells leads to further development of efficient strategies. Recently, much attention has been given to nanoparticle - based drug delivery systems loaded with dual drugs to improve current disease therapies by overcoming toxicity. In the present study, we explore an approac h to conjugate chitosan nanoparticles (CsNPs) with bee venom (BV) of Apis mellifera , then detect the enhancement of BV anticancer activity and related toxicity and anticancer potential. Chitosan nanoparticles were prepared by the ionotropic gelation method of chitosan (CS) cations with sodium tripolyphosphate (TPP) anions. Cytotoxicity of the nanoparticles against hepatocellar carcinoma (HEPG2) and Prostate cancer (PC3) was evaluated using MTT assay. Data recorded revealed that chitosan nanocapsulated ven om enhanced the toxic effect of bee venom , and flow cytometry analysis showed appreciable arrested cell cycle in the G2/M for HePG2 and PC3. Also down regulation and up regulation of Bcl2 and Bax was detected respectively in tested cell lines post treatm ent with the venom, compared with the control groups of the cancer cells respectively.
Authors and Affiliations
Walaa, A. Moselhy
Keywords
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