Evaluation of antigenotoxic activity of ethanolic extract of Calotropis procera root in 7,12-dimethylbenz[a]anthracene induced genotoxicity in Wistar rats
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 12
Abstract
Background: Chemopreventive compounds may play an important role in cancer prevention. However, many chemopreventive agents available currently are associated with toxicity. Hence, there is a need to screen for newer compounds with chemopreventive potential. Many medicinal plants can be developed as prospective chemopreventive candidates. Aims and Objectives: The aim of the study was to assess the protective effect of ethanolic extract of calotropis procera root in 7,12-dimethylbenz[a]anthracene (DMBA) induced genotoxicity in Wistar rats by micronucleus assay. Materials and Methods: Wistar rats were divided into 4 groups randomly. Group 1 received distilled water (control group). Group 2 was administered DMBA (30 mg/kg body weight [BW], single dose) intraperitoneally on the 5th day of the experiment. Group 3 rats were pretreated with C. procera root extract (500 mg/kg BW) orally for 5 days followed by DMBA injection intraperitoneally 2 h after C. procera root extract on the 5th day. Group 4 rats were given C. procera root alone orally for 5 days. The animals were sacrificed on the 6th day, and bone marrow was harvested for the micronucleus test. The percentage of micronuclei in polychromatic erythrocytes (%MnPCE) and PCE: NCE were determined. Results: Group 2 rats exhibited significant (P < 0.0001) increase in the %MnPCEs compared to control group. Group 3 and 4 rats did not show a significant increase in %MnPCEs compared to control group. The PCE: NCE ratio is decreased in Group 2 rats compared to the control group, but it is not statistically significant. The PCE: NCE ratio does not differ significantly from the control group in Groups 3 and 4. Conclusion: The ethanolic extract of C. procera root exhibits antigenotoxic activity in DMBA induced genotoxicity in Wistar rats.
Authors and Affiliations
Kunjumon Dayana, Megaravalli R Manasa
Keywords
Related Articles
- EP ID EP497838
- DOI 10.5455/njppp.2018.8.0929013092018
- Views 69
- Downloads 0
Study of psychological predictors and sleep quality in different grades of premenstrual syndrome
Background: Premenstrual syndrome (PMS) is a recurrent, cluster of symptoms and signs that develop during the 10 days before the onset of menses and subside with the onset of menstruation. It is known to affect the women...
Investigation of phytochemical constituents in Spirulina fusiformis for antibacterial activity
Background: Spirulina fusiformis provides a wide range of health benefits, so a major percentage of the population in developing countries depends on S. fusiformis due to its nutritional value. Aim and Objective: To exam...
Role of vagus in mediating the toxicity induced by Mesobuthus tamulus venom in rats
Background: Mesobuthus tumulus (red scorpion, MBT) envenomation is a serious health problem in tropical countries and is responsible for high morbidity and mortality. Aims and Objectives: Earlier reports about the role o...
Investigation of phytochemical constituents in Azolla microphylla for antibacterial activity
Background: Modern day food and lifestyle have resulted in an increasing number of diseases and disorders. A major percentage of the population depends on herbal medicines due to various needs. Aim and objective: The aim...
A cross-sectional survey on awareness of applied clinical physiology concepts among medical interns in a southern tertiary care institution
Background: In India, the preclinical subjects - anatomy, physiology and biochemistry are taught during the 1st year of medical curriculum, as per the norms of Medical Council of India. Physiology is one of the preclinic...