EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, AND ANTIFUNGAL POTENTIAL OF CUCURBITA PEPO VAR. FASTIGATA SEED EXTRACTS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 2
Abstract
Objective: The current study aims to study the antioxidant and antimicrobial and antifungal potential of the methanolic extract of Cucurbita pepo var. fastigata seeds (MECS). Methods: Extraction of the seeds has been carried out with solvents of increasing polarity (chloroform, acetone, and methanol) and the phytochemical study of the methanolic extract have been carried out using standard methods. The free radical scavenging activity of all the extracts was evaluated by DPPH and H2O2 methods. Standard disk diffusion method was used to evaluate antibacterial and antifungal activities. Results: Phytochemical evaluation showed the maximum presence of triterpenoids, phenolic compounds, tannins and small amount of Coumarins. Methanolic extract revealed momentous antioxidant activity as compared to chloroform and ethyl acetate extract. Hence, methanolic extract of C. pepo. seeds (MECS) at a dose level of 100, 200 and 300 μg/ml was evaluated for antioxidant potential. Maximum free radical scavenging activity of methanolic seed extract of cucurbita pepo var. fastigata has been found at a dose of 300 μg/ml to be 63±0.16 % by 1,1-diphenyl-2-picryl hydrazyl model and at a value of 78% at 300 μg/ml with H2O2 model. Methanolic extract also showed the presence of antibacterial activity. Conclusion: Presence of phytochemicals in the methanolic extract is responsible for the antioxidant potential. Extracts were investigated for antibacterial activity using the standard disc diffusion assay method against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and for the antifungal activities against Aspergillus niger and Candida albicans. The seed extract showed the presence of antibacterial activity, but the antifungal activity was found to be absent in the extract.
Authors and Affiliations
ROSHNI RS SONI, MANOJ BALI
Keywords
Related Articles
- EP ID EP608779
- DOI -
- Views 112
- Downloads 0
P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS
Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant wome...
A PROSPECTIVE STUDY ON DRUG UTILIZATION PATTERN OF ANTIDEPRESSANTS IN NON-PSYCHIATRIC DEPARTMENTS IN A TERTIARY CARE TEACHING HOSPITAL
Objective: The aim of this study is to evaluate the pattern of prescription and utilization of antidepressants (ADs) for various non-psychiatric indications in department of general medicine, cardiology, surgery, gynecol...
5TH INTERNATIONAL POSTGRADUATE CONFERENCE ON PHARMACEUTICAL SCIENCES 2017 (IPOPS 2017)
FACULTY OF PHARMACY UITM SELANGOR
SYNTHESIS AND INVESTIGATION OF ANTHELMINTIC, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF 3,3-DIPHENYL PROPANAMIDE DERIVATIVES
Objective: A novel series of substituted 3,3-diphenyl propanamide derivatives (I-VIII) were synthesized by reacting 3,3-diphenyl propanoyl chloride with different amines/amino acids, and all the derivatives were investig...
COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS
Objective: The objective of the present work is to isolate, purify, and fractionate anthocyanin from selected Osbeckia species and also to compare the analgesic and anti-inflammatory potentiality using animal models.Meth...