Evaluation of Antioxidant, Cytotoxic, Antimicrobial, Membrane Stabilizing and Thrombolytic Activities of Polianthes tuberose Linn
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 17
Abstract
Aims: In the present study, the crude methanol extract of tuber of Polianthes tuberosa Linn along with its all Kupchan fractions were investigated for antioxidant, cytotoxic, antimicrobial, membrane stabilizing and thrombolytic activities. Place and Duration of Study: The study was carried out for one year in 2012 in the Department of Pharmacy, Manarat International University (MIU), Dhaka-1216, Bangladesh. Methodology: The antioxidant activity was evaluated by using free radical scavenging (DPPH) assay. Here, butylated hydroxytoluene (BHT) was used as standard antioxidant. The total phenolic content was also determined and expressed in gallic acid equivalent. Cytotoxicity and antimicrobial activity of the plant fractions were determined by brine shrimp lethality bioassay as well as by the disc diffusion method, respectively. The membrane stabilizing activity was assessed by hypotonic solution and heat-induced methods and was compared with standard acetyl salicylic acid (ASA). Results: In the free radical scavenging assay, the crude methanol extraxct showed significant free radical scavenging activity with IC50 value 71.23 μg/ml. The highest phenolic content was found in crude methanol extract (113.49 mg of GAE/gm of extractives). In the brine shrimp lethality bioassay, both the crude methanol extract and its carbon tetrachloride soluble fraction demonstrated strong cytotoxic activity with LC50 value of 3.56 and 9.31 g/ml, respectively compared to that of 0.451 g/ml exhibited by standard vincristine sulfate (VS). In the disc diffusion antibacterial assay, all the plant samples showed mild to moderate activity (zone of inhibition = 9.0-15.0 mm) against test pathogens. In membrane stabilizing activity test, the plant samples at 2.0 mg/ml inhibited the isotonic solution-induced hemolysis of RBC by 65.23% and heat-induced hemolysis of RBC by 35.61%. During assay for thrombolytic activity, the crude methanol extract revealed 52.6% lysis of clot while standard streptokinase (SK) used as positive control, demonstrated 66.8% lysis of clot. Conclusion: The plant possesses significant bioactivities which rationalize its use as folk medicine.
Authors and Affiliations
Farhana Rumi, Md. Ruhul Kuddus, Sujan Chandra Das
Keywords
Related Articles
- EP ID EP344649
- DOI 10.9734/BJPR/2014/12414
- Views 95
- Downloads 0
Batch Equilibrium, Kinetics and Thermodynamics Study of Sulfamethoxazole Antibiotics Onto Azolla filiculoides as a Novel Biosorbent
Azolla filiculoides (AF) were used as adsorbent for the sorption of Sulfamethoxazole (SMZ) from aqueous solution. Also adsorption Isotherms, kinetics and thermodynamics were studied. Experiments were conducted by varying...
Formulation and Characterization of Nystatin-loaded Nanostructured Lipid Carriers for Topical Delivery against Cutaneous Candidiasis
Aims: The objective of the current study was to formulate nystatin (Nyst) into nanostructured lipid carriers (NLCs) to enhance its antifungal activity. Place and Duration of Study: Department of pharmaceutical technolog...
Molecular Modeling of the Major Compounds of Sesquiterpenes Class in Copaiba Oil-resin
Aims: To study the Structure-Activity Relationship (SAR) and pharmacokinetic and toxicological properties (ADME/Tox) of the major compounds of sesquiterpenes class oil-resin extracted from the copaiba, using quantum chem...
A Comprehensive Review on Bioequivalence Studies in Human Subjects
Aims: Purpose of the current review was to provide information about the bioequivalence and the bioequivalence study steps in human subjects to conduct bioequivalence studies easily as well as to increase the use of alte...
Bioactive Constituents from Apium leptophyllum Fruits
Aims: This study was undertaken to characterize the bioactive constituents of Apium leptophyllum fruits and to evaluate their cytotoxicity, anti-inflammatory and alpha-amylase inhibitory effects. Study Design: Isolation...