EVALUATION OF CARTRIDGE BASED NUCLEIC ACID AMPLIFICATION TEST IN DIAGNOSIS OF PULMONARY TUBERCULOSIS
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 74
Abstract
BACKGROUND Tuberculosis still continues to be one of the commonest cause of infectious disease related morbidity in the developing world. Diagnosis of pulmonary tuberculosis is mostly relied on sputum smear microscopy and sputum for mycobacterial culture. In recent years, cartridge based nucleic acid amplification test (CBNAAT) has been recommended by World Health Organization as a rapid diagnostic test for detection of tuberculosis and rifampicin resistance. The aim of this study is to determine the diagnostic yield of CBNAAT in pulmonary tuberculosis (PTB) and to compare its efficacy between sputum smear-positive and sputum smear-negative pulmonary tuberculosis. MATERIALS AND METHODS A prospective observational study was conducted in the Department of Respiratory Medicine in a teaching hospital in eastern India for a period of one year. All re-treatment cases of PTB, newly detected smear-negative PTB and all human immunodeficiency virusPTB co-infected cases were recruited for the study and sputum for CBNAAT was performed in all of them. Results were analysed in light of diagnostic yield of CBNAAT with special emphasis on comparing CBNAAT results between different subgroups- sputum smear-positive, sputum smear-negative PTB and immunocompromised patients. RESULTS Out of 228 cases of PTB, 190 were sputum smear negative and 38 cases were sputum smear positive. Mean age of the study population was 33 ± 17.13 years. Sensitivity of sputum smear for AFB was 16.67% (CI-12.07%-22.15%) with a negative predictive value (NPV) of 20.83% (CI- 19.89%-21.81%). Sputum smear negativity was found to be more common in females. CBNAAT was positive in 111 cases. Overall, sensitivity of CBNAAT was 48.68% (95% CI- 42.03%-55.37%). Sensitivity of CBNAAT varied significantly between sputum smear-positive PTB (100%, CI- 92.89%-100%) and sputum smear-negative PTB (38.42%; CI31.47%-45.74%) (p- <0.0001). Sensitivity of CBNAAT was 33.33% (CI-17.96%-51.83%) in PTB-HIV co-infected patients. Rifampicin resistance was detected in five (2.2%) patients with 100% sensitivity and specificity. CONCLUSION CBNAAT adds significantly to the diagnostic yield of PTB in comparison to sputum smear microscopy, but its sensitivity is lower in sputum smear-negative cases. It has additional advantage of identifying rifampicin resistance with high sensitivity and specificity.
Authors and Affiliations
Subhasis Mukherjee, Debabani Biswas, Shabana Begum, Priyanka Ghosh, Amitava Paul, Supriya Sarkar
Keywords
Related Articles
- EP ID EP265871
- DOI 10.14260/Jemds/2017/1147
- Views 62
- Downloads 0
POWERPOINT PRESENTATION VS BLACKBOARD TEACHING: A COMPARATIVE STUDY AND EVALUATION IN GOVERNMENT MEDICAL COLLEGE, ANANTHAPURAMU, ANDHRA PRADESH FOR II M.B.B.S STUDENTS: A QUESTIONNAIRE BASED STUDY
Chalk talk lecture is the oldest and most commonly used teaching technique. However, it is also common to use PowerPoint presentations after the introduction of digitalized teaching in medical colleges. This stu...
INCIDENTAL LEIOMYOMA IN MESH HERNIOPLASTY
Incidental finding of leiomyoma is rare finding and can mimic an inguinal hernia or both can occur at a time. Very small mass beside hernia sac identified during exploration of the inguinal canal. Inguinal hern...
COMPARATIVE EVALUATION OF BOLUS ADMINISTRATION OF ESMOLOL VERSUS LIGNOCAINE FOR PRESSOR RESPONSE ATTENUATION DURING LARYNGOSCOPY AND ENDOTRACHEAL INTUBATION
BACKGROUND Endotracheal intubation and anaesthesia have become an integral part of the anaesthesiologist’s contribution to the patient care. Endotracheal intubation is the translaryngeal placement of endotracheal tube in...
INTESTINAL PHYTOBEZOAR
PRESENTATION OF CASE A 19-year-old naval cadet presented with abdominal pain of 03 hours duration. Pain was of sudden onset confined to the periumbilical region. Pain was colicky in nature without relieving or aggravatin...
PEDUNCULATED VULVAL AGGRESSIVE ANGIOMYXOMA: A DIAGNOSIS OFTEN OVERLOOKED
Aggressive angiomyxoma is a rare, locally infiltrative mesenchymal tumor found usually in women of reproductive age group. It is a slow-growing, low-grade neoplasm involving the pelvis and perineum with a high risk...