Evaluation of Chemokine Profile in Stored Whole Blood
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 6
Abstract
Bizarre immunological changes have been known to occur when whole blood is stored. This study investigated the changes that could occur in chemokine profile in whole blood stored in CPDA-1 anticoagulant/preservative for 28 days. This was an observational longitudinal study. The study was carried out at Nigerian National Petroleum Corporation Medical services, Akpajo, Rivers State. The effect of storage on these parameters were assessed and compared with a control. A total of 50 samples divided into 5 sets (1, 7, 14, 21, 28 days) of 10 samples each, gotten from 10 apparently healthy voluntary donors with day 1 samples used as control. The blood donors were between the ages of 24 to 57 years. Chemokine (IL-6, IL-8, IL-10 and TNF-α) were analyzed with reagents from Elabscience, Nuhan, China, using ELISA machine. The mean chemokine values for Day 1 and Day 28 were respectively IL-6: 14.61 ± 8.88pg/ml and 1064 ± 232.8pg/ml IL-8: 46.96 ± 8.32pg/ml and 769 ± 196.3pg/ml IL-10: 18.45 ± 14.56pg/ml and 1101 ± 249.4pg/ml and TNFα: 30.38± 26.52 pg/ml and 1096 ± 281.4 pg/ml. There was a significant increase in the mean values of chemokines at P-values <0.0001.Increase in the concentration of chemokines (IL-6, IL-8, IL-10 and TNFα) could probably be due to WBC degradation, which could induce pro-inflammatory response upon blood transfusion. It is therefore necessary to put into consideration the need for whole blood to undergo leukodepletion before storage and if it must be stored beyond one week. .
Authors and Affiliations
T. C. Adias
Keywords
Related Articles
- EP ID EP513005
- DOI -
- Views 68
- Downloads 0
A Cross Sectional Study to Evaluate the Psychosocial and Health Challenges in School Students
Background: Students face many situations in school that may result in psychosocial and health problems and rather than for the school to be a source of positive socialization. The present study was aimed at determining...
Histogenesis of Human Fetal Thymus in 1st and 2nd Trimester
The thymus is the lymphoid organ that consists of lymphoid and epithelial cells The thymus is composed of two identical lobes and is located anatomically in the anterior superior mediastinum. In spite of remarkable progr...
Role and Prevention of Tobacco for Oral Health Care. Where Are We Now???
Tobacco use in India continues to promote various diseases and kill several individual but still no significant steps are been taken to get rid of this threat. Not only cancer but tobacco plays as the key factor for vari...
Reproductive Performance of Women with Fibroids
The association between fertility and fibroid is known, however a cause-effect relationship is not established. Majority of studies are from infertility centers or referral hospitals. A retrospective study was undertaken...
A Rare Case Report on Use of IABP in Post Op-Mediastinal Mass Removal
A 50-year-old female patient, presented with mediastinal mass (H/P thymoma) with total occlusion of the right main bronchus. After removal of the mass, sudden dilatation of LV and Lv failure was occurred. High inotropic...