Evaluation of Commercial Rapid Diagnostic Test Kit for Tuberculosis: Further Evidence Supporting Negative Policy on the Use of Serological Tests for Pulmonary Tuberculosis Diagnosis in Developing Countries
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2014, Vol 4, Issue 2
Abstract
Aim: To know whether one of the commercially available immunochromatographic tuberculosis tests is comparable with the widely available method, direct sputum microscopy. Design: The study prospectively validated the pulmonary tuberculosis rapid test kit using the reference standard, Lowenstein Jensen culture and compared the outcome with the direct sputum microscopy. Place and Duration: The study was conducted in Zankli Medical Centre, Abuja, between November 2004 and July 2005. Methodology: 340 patients from direct observation therapy clinics located in six different government owned health facilities were referred to our facility. These patients; male (192) and female (148) were between the age of 10 and 64 years old. Three sputa samples were collected over two consecutive days and direct microscopy and culture were performed on these samples. Also, 4ml of blood were collected from the same patients for antibody detection using immunochromatographic technique. Results: The evaluated rapid diagnostic kit when compared with the reference standard has a sensitivity of 59.3% and 81.1% specificity. Sensitivity and specificity of direct microscopy, when compared with the rapid test is statistically significant (P=0.001); indicating diagnostic accuracy of the conventional method of pulmonary tuberculosis testing over the immunochromatographic test. Conclusions: The conventional test indicated high performance in this report and it is suggestive of the relevance and diagnostic accuracy of the widely available method in the diagnosis of pulmonary tuberculosis in developing countries. This assertion is also, supported by the 2008 WHO/TDR report on evaluation of nineteen tuberculosis rapid diagnostic kits.
Authors and Affiliations
Ameh James, Kingsley Ochei, Nnamdi Emenyonu, Saffiatou Darboe, Lovett Lawson
Keywords
Related Articles
- EP ID EP348597
- DOI 10.9734/BJMMR/2014/5802
- Views 37
- Downloads 0
The Relationship between TV Viewing and Food Intake and BMI in Preschool Children
Background: Considerable attention is currently being paid to childhood nutrition. Mass media, particularly television (TV), is believed to largely contribute to eating habits and Body mass index (BMI). This study was ca...
Serial DXA Bone and Soft Tissue Estimations in Growing Sheep
Objectives: We aimed to establish the typical rate of skeletal mineralisation in the growing sheep. The sheep (size, ease of handling, cost and bone physiology) is establishing itself as an appropriate model for bone res...
A Review of Efficacy of Hippotherapy for the Treatment of Musculoskeletal Disorders
Objective: Hippotherapy or Equine Assisted Therapy (EAT) is an alternative therapeutic treatment which is based on the special benefits of horse riding. The purpose of the herein review is to investigate the efficacy of...
The WHO FRAX Calculator with and without BMD as an Indicator of Low BMD in Pakistani Women
Aims: BMD (bone mineral density) testing facilities are still scarce in Pakistan. We evaluated the predictive ability of FRAX with BMD (FRAX+) and FRAX without BMD (FRAX-) in a set of Pakistani females to assess their ro...
The Association of Eating Attitudes between Metabolic Control and Quality of Life in Patients with Type 1 and Type 2 Diabetes Mellitus
Aims: The combination of an eating disorder and diabetes mellitus (DM) puts patients at high risk of mortality and morbidity. The purpose of this study was to determine the association of eating attitudes between metabol...