Evaluation of CYP1B1 Expression, Oxidative Stress and Phase 2 Detoxification Enzyme Status in Oral Squamous Cell Carcinoma Patients
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 3
Abstract
Introduction: Oral Squamous Cell Carcinoma (OSCC) affects global health with increasing incidence and mortality rate. It may involve exposure to carcinogens from tobacco smoking or chewing. Alteration in expression of gene encoding the enzymes concerned with carcinogen biotransformation along with oxidative stress may increase or decrease the risk of cancer. Aim: To evaluate the expression of CYP1B1 gene in OSCC patients along with its relation with oxidative stress and phase 2 detoxification enzyme status. Materials and Methods: In the present study, CYP1B1 genotypic analysis was carried out along with estimation of serum Total Antioxidant Activity (TAA), erythrocyte Malondialdehyde (MDA) levels and serum Glutathione-S-Transferase (GST) activity in 20 OSCC patients and statistically compared with that of age matched 20 healthy subjects, served as control by using student’s t-test. Results: It was observed that 85% of histopathologically diagnosed OSCC patients had CYP1B1 expression with significantly elevated levels of MDA (p<0.001). In addition, plasma total antioxidant status and serum GST levels were decreased significantly (p<0.05) in OSCC patients as compared to the healthy controls to overcome the burden of oxidative stress. Conclusion: On the basis of the present study, we conclude that the expression of CYP1B1 is an important determinant of carcinogenesis and significantly associated with oxidative stress characterized by decreased serum GST and total antioxidant levels in OSCC patients.
Authors and Affiliations
Shailza Verma, Rahul saxena, Merajul Haque Siddiqui, Karunathy Santha, Subramaniam Sethupathy
Keywords
Related Articles
- EP ID EP333831
- DOI 10.7860/JCDR/2017/22196.9324
- Views 94
- Downloads 0
Effective Treatment of Epithelial Downgrowth in Persistent Wound Leak Following Removal of Silicone Oil
This case report aims to share the successful management of Epithelial Downgrowth (ED) which occurred following persistent wound leak, after Removal of Silicone Oil (ROSO) via the anterior chamber. A teenage male with hi...
Analysis of Survival Data: Challenges and AlgorithmBased Model Selection
Survival data is a special form of time to event data that is often encountered while modelling risk. The classical Cox proportional hazard model, that is popularly used to analyse survival data, cannot be used for model...
Focal Adhesion Kinase Expression in Ameloblastoma: A Preliminary Observational Study
Introduction: Focal Adhesion Kinase (FAK) is a membraneassociated molecule, which plays a major role in tumourigenesis by promoting cell survival, proliferation, migration, and invasion. Hence, FAK is a well known molecu...
Isolated Renal Hydatid Disease-A Cytohistological Diagnosis
ABSTRACT Hydatid disease caused by Echinococcus granulosus is a common occurrence in liver and lungs. Isolated renal hydatid cyst without liver or lung involvement is extremely rare. We present a case of solitary renal h...
Hemangiopericytoma: A Rare Mass Arising in the Kidney
ABSTRACT Hemangiopericytoma, a tumour arising from pericytes is an unusually rare kidney neoplasm. It is difficult to establish a preoperative diagnosis. Here, we present a case report of a 47-year-old gentleman who pres...