EVALUATION OF CYTOTOXICITY AND GENOTOXICITY OF Micromeria pulegium (Rochel) BENTH EXTRACT IN HUMAN LYMPHOCYTES AND GR-M MELANOMA CELLS IN VITRO
Journal Title: Genetics & Applications - Year 2018, Vol 2, Issue 1
Abstract
Micromeria pulegium (Rochel) Benth is an endemic species of Lamiaceae family that includes plants frequently used in culinary and folk medicine. As cytotoxic potential of some species of Micromeria genus has been confirmed, this study aimed to test unknown antiproliferative and genotoxic potential of aqueous leaf extract of M. pulegium, endemic bh. species, in normal (human lymphocytes) and cancer (human melanoma GR-M) cells. The results of study would aid the burden of evidence required for the establishment of conservation program of small populations of native M. pulegium or promote its controlled micropropagation and/or cultivation. Cytokinesis-block micronucleus cytome assay was applied to human lymphocyte cultures, while trypan blue exclusion assay was used for evaluation of cytotoxicity in human GR-M melanoma cells. No genotoxic effects were detected in human lymphocyte in vitro up to the concentration of 0.2 mg/ml but cell viability in human GR-M melanoma cell line cultures treated with 0.3 mg/ml of Micromeria aqueous extract was significantly reduced.
Authors and Affiliations
Anja Haverić, Jasmina Čakar, Maida Hadžić, Sanin Haverić
Keywords
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- EP ID EP349560
- DOI 10.31383/ga.vol2iss1pp25-31
- Views 112
- Downloads 0
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