EVALUATION OF DIAGNOSTIC UTILITY OF USING TWO SUBSTRATES FOR ENDOMYSIUM ANTIBODIES TEST WITH COMPARISON WITH HISTOLOGICAL FINDINGS IN IRAQI COELIAC PATIENTS
Journal Title: International Journal of Medical and Clinical Research - Year 2012, Vol 3, Issue 3
Abstract
Purpose: A case-control study was carried out to elucidate prospectively the sensitivity and specificity of endomysium antibodies by using two substrates in the diagnosis of coeliac disease as a first attempt in an Iraqi population with comparison with histological findings. Methods: The study consisted of 314 patients and 100 normal controls. Oesophago-gastroduodenoscopy was done to each patient and three biopsies from distal duodenum were taken. Venous blood sample was also obtained from each patient and normal subjects. The sera were tested for endomysium antibodies (EMA) by using two substrates; monkey esophagus and human umbilical cord by indirect immunofluorescent technique (IIF). Results: The sensitivity of EMA (monkey oesophagus) test among both children and adults was 93.8% and 100% respectively, while the sensitivity of EMA (umbilical cord) test among children and adults was 96.6% and 100% respectively. Conclusions: The human umbilical cord tissue is better for using as a substrate than the monkey esophagus tissue among Iraqi ceoliac patients. Therefore, the test is suitable as a diagnostic and screening tool for the disease.
Authors and Affiliations
MUHAMED T. OSMAN, GHADA AL-DUBONI, SANA A A AL-NASIRY, H. F. MAKKI, I. T. BALSAM
Keywords
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- EP ID EP170049
- DOI 10.9735/0976-5530.3.3.127-131
- Views 96
- Downloads 0
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