Evaluation of Different Diagnostic Techniques for Detection of Cryptosporidium spp. in Faecal Specimens
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 9
Abstract
Abstract: Cryptosporidium spp. is a 4-6 µm coccidian parasite causing mild to fulminant diarrhoea especially in immunocompromised persons. It remains largely under-diagnosed by using current routine diagnostic techniques in microbiology laboratories. The aim of our study was to compare four different diagnostic techniques for the detection of Cryptosporidium spp. in faecal specimens in cases of both acute and chronic diarrhoea. The present study was carried out in the Department of Microbiology for the detection of Cryptosporidium spp.in stool samples collected from 177 children suffering from diarrhoea. All the stool samples were examined microscopically after concentration by the formol-ether sedimentation technique. Sediments were examined by iodine-stained wet mount preparations and were stained with two staining techniques – Modified ZiehlNeelsen(Z-N) and Safranine methylene blue staining techniques. Samples were further subjected to Enzyme Linked Immunosorbent Assay (ELISA).Chi-square and z tests were used to compare differences between the groups. A p value of ≤ 0.05 was considered significant. ELISA gave the highest rate of positivity (12.99%) followed by Safranine methylene blue staining and Z-N staining in which the yield was 11.9%. The wet mount preparation technique yielded the lowest number of positive samples (9%). We considered cryptosporidiosis to be a definite diagnosis if the organisms were found in any two of the four techniques. The overall prevalence of Cryptosporidiumspp.was found to be 11.9%.Both the conventional staining methods and ELISA had similarities in sensitivity and sensitivity patterns. The conventional staining methods were found to be more cost-effective in comparison to ELISA but were found to be time-consuming, labour-intensive and required greater skill and experience. Keywords:evaluation, diagnostic methods, cryptosporidium, children, diarrhoea.
Authors and Affiliations
Farhat Tahira, Amit Kumar Singh, aiya ba
Keywords
Related Articles
- EP ID EP371508
- DOI -
- Views 54
- Downloads 0
A Study on Cardiac Rhythm Abnormalities in the First 48 Hours Following Acute Myocardial Infarction
Acute myocardial infarction is a major public health problem in developing countries. The incidence of coronary artery disease varies from 20-30 % of the cardiac cases admitted in any general hospital. Cardiac arrhythmia...
Comparison of five different modalities in diagnosis of coronary artery disease
This is a hospital based study comparing the five most common diagnostic modalities for coronary artery disease, ECG, ETT, Rest and Stress Echo, 99mTc-MIBI, and Angiography, taking Angiography as the gold standard. Seven...
Feasibility and Benefits of Laparoscopic Sigmoidectomy Versus Open Sigmoidectomy, for Sigmoid cancer
Laparoscopic sigmoidectomy has been shown to have benefits compared to open. The incorporation of laparoscopy in colon surgery is challenging, due to the high cost of equipments. The aim of this study was to evaluate t...
Bimalleolar Fracture of Ankle Joint Managed By Tension Band Wiring Technique: A Prospective Study
Ankle fractures are the most commonly encountered by most of the orthopaedic surgeons. According to the lauge Hansen’s classification five different types can be seen. The surgical treatment of adduction, abduction and s...
The Effect and Benefits of Pet Facilitated Therapy (PFT) in the Elderly
Abstract: With the advanced age, the elderly people are facing undergoing decline in their physical, mental and social aspects. Alzheimer, Dementia, and Cognitive function disorders are among the common health problems w...