Abstract

Background: Escherichia coli (E.coli) is most common organism responsible for UTI because of its various virulence factors to break the inertia of mucosal barrier. Objective: In this present study we aimed to access the different virulence factors and their correlation with multi-drug resistance. Materials and Methods: This was a prospective study done at Burdwan Medical College and Hospital, West Bengal. A total 100 urine samples from clinically suspected UTI patients were processed by standard microbiological procedures. Fifty (50) E.coli were isolated on MacConkey agar showing significant bacteriuria from the 100 samples studied. The virulence factors studied were Haemolysis, Bio-flim formation, Mannose sensitive/ resistant haemagglutination, serum resistance and antibiotic resistance pattern. For bio-film three methods – congored agar, tube method and tissue culture plate method were compared. Haemolysis was observed on sandwich blood agar. Mannose resistance and sensitive haemagglutination and serum resistance were determined by standard methods. ESBL and MBL were detected by double disc diffusion method along with conventional antibiogram. Result: Fifty E.coli were isolated from 100 urine sample in this study. Among these 30% showing haemolytic properties, 48% showing HA and 16% showing serum resistance. Three different methods of bio-film formation were evaluated. Antibiogram was plotted with MDR. Conclusion: There is a need of periodic survillence, antibiotic policy, careful use of empirical and target antibiotics, use of antibiotic coated catheter, standard guidelines on care of catheter. This will reduce incidence, chronicity and recurrence of Urinary Tract Infections.

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