Evaluation of DNA damage levels in Oral leukoplakia by Single Cell Gel Electrophoresis Assay
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 4
Abstract
: Comet assay also known as Single cell gel electrophoresis (SCGE) assay is a rapid and highly sensitive fluorescent molecular technique for detecting various forms of deoxyribonucleic acid (DNA) damage at individual cellular level. The present study was done to detect the extent of DNA damage in oral leukoplakia (OL) and compare with normal individuals. The sample population was obtained from Department of Dental Surgery, Sri Padmavathi Medical College for women, and Sri Venkateswara Institute of Medical Sciences (SVIMS) Tirupathi. Andhra Pradesh. In the period of 24months,from January 2017 to January 2019. A total of 180 consecutive patients with leukoplakia and 20 healthy normal volunteers were recruited for the study and assessed for the extent of DNA damage using SCGE following clinical diagnosis and histological grading. Peripheral blood was obtained by venipuncture and SCGE assay was performed. Mean comet tail length was recorded and analyzed statistically to compare the extent of damage in study subjects. The mean comet tail length seen in leukoplakia patients 1.65 ± 0.132 µm while for the control subjects, it was 0.42 ± 0.14 µm. The difference was statistically significant. On comparing within the leukoplakia and Control, a progressive trend of increasing tail length was observed with increasing in study subjects. Deoxyribonucleic acid damage as measured by SCGE was seen in leukoplakia. A stepwise increase in DNA damage levels from healthy controls, through patients have been observed indicating the extent of DNA damage in this study group.
Authors and Affiliations
Dr. P. Vani Priya MDS, Dr. G. Kiranmai MDS
Keywords
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- EP ID EP572553
- DOI 10.9790/0853-1804065659.
- Views 62
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