EVALUATION OF DRUG YIELDING POTENTIAL OF MICROPROPAGATED CURCUMA AROMATICA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: GC MS analysis and antioxidant activity of micropropagated and conventionally grown Curcuma aromatica essential oil and extract was done for their large scale commercial cultivation. Molecular marker based studies were performed to know their genetic fidelity as well as to trace any somaclonal variation existing between the regenerants.Methods: In vitro regeneration and multiplication were done using Murashige and Skoog media with various combinations of growth regulators. Component identification was done by GC MS analysis. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used for molecular profiling. Antioxidant activity was performed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH).Results: Molecular marker-based analysis revealed uniform banding patterns similar to those of the mother plants. Gas chromatography and mass spectroscopy (GC MS) analysis showed the presence of 10 major components accounted for 95.5% of the total compounds. The major components in micropropagated and field grown mother plants were found to be alpha phellandrene (41% and 38%), 4-carene (23% and 25%) and terpeneol etc. Antioxidant activity of leaf oil (IC50-29.3 µg/ml) and methanolic extract (IC50-101.25 µg/ml) of in vitro grown plants showed increased free-radical scavenging activity. Conclusion: Absence of any type of remarkable polymorphism in the essential oil quality and antioxidant activity the present protocol could be used commercially for large scale propagation of C. aromatica. The present report bears immense potential for the future improvement, conservation and domestication of C. aromatica to explore its high prized secondary metabolites.Â
Authors and Affiliations
Sujata Mohanty, Reena Parida, I Sriram Sandeep, Suprava Sahoo, Sanghamitra Nayak
Keywords
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