EVALUATION OF EARLY LYMPH NODE METASTASIS IN SQUAMOUS CELL CARCINOMA OF THE PENIS WITH THE AID OF p53 IMMUNOMARKER
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2019, Vol 6, Issue 11
Abstract
BACKGROUND Use of p53 as a prognostic marker to detect early lymph node metastasis in carcinoma of penis in order to avoid unnecessary lymphadenectomy was evaluated. The aim of the study is to detect early metastasis in amputated specimens of penis, by using p53 immuno marker, and correlation with the histopathological variables and clinical stage. MATERIALS AND METHODS Retrospective study of 49 cases of partial /total amputation with lymphadenectomy of penile cancers were divided into metastatic and non-metastatic groups on H&E sections in which 16/49 had metastasis. Histological slides from all cases were processed with immunohistochemical technique using anti‑p53 antibodies. The p53 tumour density was calculated in 100 cells with 20% cut off and was graded as positive when more than 20% of the tumour cells showed positive nuclear staining. All the pathological and clinical variables were calculated by chi square test. RESULTS 13/49 cases showed increased p53 tumour density, p-value of 0.05 was considered significant in our study. p53 did not correlate statistically with histopathological and clinical variables like phimosis, BXO, clinical node status, tumour type, tumour grade, depth of invasion, LVI. p53 had low positive predictive value. Therefore, in our study, p53 cannot predict the early lymph node metastasis because of low positive predictive value, even though they are sensitive. CONCLUSION p53 immunomarker had low specificity and low positive predictive value, which suggests that it is not able to predict early lymph node metastasis even though it is sensitive and thus it is not helpful in deciding for prophylactic lymphadenectomy.
Authors and Affiliations
Miriam Sandeep Eden, Ramani Kumar
Keywords
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- EP ID EP615531
- DOI 10.18410/jebmh/2019/175
- Views 89
- Downloads 0
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