Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)
Journal Title: JOURNAL OF ADVANCES IN AGRICULTURE - Year 2018, Vol 8, Issue 1
Abstract
Coffee is one of the most important cash crops cultivated in the world with great economic importance. During the induction of somatic embryogenesis, there are different endogenous compounds involved in the success or failure of the somatic embryogenesis response and these compounds determine the specificity of cellular responses. This present experiment identified and quantified endogenous sugars, chlorogenic acid and caffeine present during somatic embryogenesis of ‘Ruiru 11’. Laboratory experiments were set up at Coffee Research Institute, Ruiru-Kenya between 2014 and 2016. Third leaf pair explants were excised from 8-monthold greenhouse-grown mother plants and cultured in half strength Murashige and Skoog basal salts augmented with Thidiazuron. Once embryos had developed, the cultures were analysed for endogenous sugars, caffeine and chlorogenic acid using HPLC. Generally, green embryogenic cultures contained more and higher quantities of the compounds. Glucose and fructose were highest (38.95 mg/g and 45.43 mg/g respectively) in leaf discs of brown non-embryogenic cultures. Sucrose was highest (62.15 mg/g) in embryos of green embryogenic cultures. Embryos of green embryogenic cultures had the highest chlorogenic acid (5.3 mg/g), whereas caffeine was highest (0.55 mg/g) in embryos of brown embryogenic cultures. Endogenous fructose and glucose inhibited embryogenesis, while sucrose, chlorogenic acids and caffeine promoted embryogenesis and are potential biomarkers for embryogenesis. Other biochemical compounds such as organic acids should be identified and their role in coffee somatic embryogenesis determined.
Authors and Affiliations
D. K. Isutsa, R. N. Mayoli, A. B. Nyende, C. M. Mweu
Keywords
Related Articles
- EP ID EP652466
- DOI 10.24297/jaa.v8i1.7376
- Views 132
- Downloads 0
FARMERS PERCEPTION AND THE HIGHLIGHTS OFTHE SOURCES OF THE CHRONIC AFLATOXIN CONTAMINATION AMONGST THE INHABITANTS OF THE SOUTH-EASTERN REGION INKENYA
There are factors that contribute to aflatoxin contamination like conducive ecological zones, stressed crops, virulent Aspergillus strains and unconventional agricultural practices. Kenyans especially in the South-Easter...
Welfare of a pair of Captive Tigers: a Hand-Reared Female and a Parent-Reared Male
Animal welfare assessment has undergone considerable development. The management and rearing of big cats may lead these animals to express behavioral problems. This study was performed to assess the well-being of a non-b...
Germination and initial growth of triticale seeds under stationary magnetic fields
The main objective of this study is to determine the effects of 125 mT and 250mT magnetic treatment on the germination and initial growth of triticale seeds. This objective has a practical application in agriculture scie...
Pathogens of forrest trees in nurseries a minireview
The forest trees nurseries are attacked by different pathogens which are causing important damages and economic loss in forest commercial exploitations. One of the most important losses are in the tree nurseries, damagin...
The Mechanism Behind the Promotive Effect of Foliar Application of 5-Aminolevulinic Acid (ALA) in Tomato Plants Under Salt Stress
The effect of foliar ALA application on the internal ALA concentration in tomato plants grown in soil containing high levels of NaCl was investigated. Six week old plants were treated with 100, 50, and 25 mmol/L Na...