Evaluation of immature monocyte-derived dendritic cells generated from patients with colorectal cancer
Journal Title: Polish Journal of Surgery - Year 2013, Vol 85, Issue 12
Abstract
Dendritic cells are heterogeneous population of the leukocytes and most potent APC in activation of naive T lymphocytes. Therefore the DCs generated in vitro are under research for their application in anti-tumor immunotherapy. The aim of the study was generation of the immature dendritic cells from peripheral blood monocytes collected from colorectal cancer patients and comparison of their ability to endocytosis, cytokine production and immunophenotype to DCs generated from healthy donors. Material and methods. 16 adenocarcinoma stage II patients were included in the study. Dendritic cells were generated in the presence of rhGM-CSF and IL-4. PBMC were isolated from the blood of patients and 16 healthy donors – control group. Immunophenotype, ability of endocytosis of DextranFITC as well as intracellular IL-12 expression of the generated dendritic cells was measured using flow cytometry. The cytokines (IL-6, IL-10, IL-12p70, IFN-γ) concentration in the supernatants of DCs culture was measured by ELISA. Results. The percentage of the immature dendritic cells and expression of CD206 and CD209 antigens was significantly higher in patients group (p <0.05 and p <0.001 respectively). Significantly (p <0.001) higher expression of the antigens which initiate the Th2 immune response (CD80-/CD86 + and B7-H2 + / CD209 +) was in the patients group. There were no differences in endocytosis ability and the cytokines (IL-6, IL-10, IL-12p70, IFN-γ) concentration between investigated groups. Conclusions. High immature markers expression on the generated dendritic cells together with identical endocytosis ability in patients group is advantageous in antitumor autologous cells immunotherapy planning. However there is one troubling fact – high expression of markers, which may induce tolerance to particular antigen. It seems to be more reasonable to use the autologous DCs in the antitumor immunotherapy, especially due to the incompatibility in allogenic cells in the context of HLA complex.
Authors and Affiliations
Ryszard Maciejewski, Sebastian Radej, Jacek Furmaga, Andrzej Chrościcki, Sławomir Rudzki, Grzegorz Wallner
Keywords
Related Articles
- EP ID EP74528
- DOI 10.2478/pjs-2013-0109
- Views 124
- Downloads 0
Laryngektomia całkowita w leczeniu raka krtani w stopniu zaawansowania T4: trendy i ocena czasu przeżycia
Wprowadzenie: Postępowanie w zaawansowanym raku krtani ulegało zmiennym trendom w ciągu ostatnich kilku dekad. Chociaż w literaturze dostępnych jest wiele obszernych doniesień na temat wyników przeżycia w przypadku zaawa...
The use of the enhanced recovery after surgery (ERAS) protocol in patients undergoing laparoscopic surgery for colorectal cancer – a comparative analysis of patients aged above 80 and below 55
Age is one of the principal risk factors for colorectal adenocarcinoma. To date, older patients were believed to achieve worse treatment results in comparison with younger patients due to reduced vital capacity. However,...
Portal Vein Resection During Pancreatectomy for Pancreatic Head Adenocarcinoma. Scope of Current Opinions and own Experiences
Peritoneal Adhesions as a Cause of Mechanical Small Bowel Obstruction Based on Own Experience
Bowel obstruction is a condition which has been known for many years. As time goes by, the problem is still often encountered at surgical emergency rooms. More than 20% of emergency surgical interventions are performed b...
Initial experience with endoscopic sleeve gastroplasty in Poland
Introduction: Obesity is becoming one of the major public health problems. Bariatric procedures are considered the most effective methods of treating this condition but they are costly and entail a high risk of complicat...