Evaluation of In-House Multiplex Polymerase Chain Reaction (MPCR) For Diagnosis of Pulmonary Tuberculosis
Journal Title: Journal of Medical Science And clinical Research - Year 2016, Vol 4, Issue 4
Abstract
Background: Diagnosis of tuberculosis is still an ongoing problem however nucleic acid amplification test has emerged as a promising tool for its diagnosis. Single gene target may result in false negative due to the absence or the presence of only few copies of target DNA in some M.tuberculosis isolates. The objective of this study was to evaluate a multiplex PCR (MPCR) for the detection of M. tuberculosis in sputum samples. Materials & Methods: 150 clinically suspected cases of pulmonary tuberculosis were processed for detection of mycobacterial infections by ZN staining smear examination, LJ culture and Multiplex PCR tests which comprised of genus specific primer targeting insertion sequence 6110 (IS6110), MPB64 (23kDa) and Protein b (38kDa). Results: Multiplex PCR showed highest sensitivity of 100 %, followed by 65.2 % for AFB smear when LJ culture was considered as gold standard. Conclusion: Multiplex PCR increased the sensitivity and it can be used to detect samples with M.tuberculosis strains lacking IS6110
Authors and Affiliations
Dr Dakshina Bisht
Keywords
Related Articles
- EP ID EP209438
- DOI -
- Views 69
- Downloads 0
Cervical Smear Screening Using Cost-effective Liquid Based Cytology
Objective: To minimise false negativity in cervical cancer screening with Papanicolaou (Pap) test, there is a need to explore novel cytological technique and identification of unique and important cellular features. Meth...
Prevalence of Hypothyroidism in Major Psychiatric Disorders in Patients Attending Outpatient Department
Background: Defecieny of thyroid hormone leads to hypothyroidism. There is relationship between the major psychiatric disorders and hypothyroidism. Objective: To determine the prevalence of hypothyroidism in patients att...
Comparative Study of Radiological Features of Pulmonary Tuberculosis in HIV Infected Patients in Correlation with CD4 Counts
Tuberculosis, an ancient disease, and a major public health problem which is now complicated by relentless spread of HIV & AIDS pandemic and the emergence of multi drug resistant strains.HIV and TB, each disease speeds u...
Screening of Aminoacidurias in Children
Inborn errors of metabolism (IEM) are genetically determined biochemical disorders due to specific congenital defects in protein molecules. In infants and young children the inborn errors produce mental deficiency and se...
Assessment of treatment with Intravesical alkalinized lidocaine and heparin in Painful bladder syndrome /Interstitial cystitis (PBS/IC)
Introduction: Painful bladder syndrome or Interstitial cystitis (IC) is characterized by an unpleasant sensation (pain, pressure, and discomfort) perceived to be related to the urinary bladder and presented as urgency, f...