Evaluation of in vitro Anti-proliferative Activity of L-arginine deiminase from Novel Marine Bacterial Isolate
Journal Title: Microbiology Research Journal International - Year 2016, Vol 13, Issue 5
Abstract
L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activity of the purified l-arginine deiminase from Vibrio alginolyticus 1374. Production of the enzyme was carried out by shake flask method under optimal conditions. The enzyme thus produced was purified to near homogeneity by ammonium sulphate fractionation followed by ion exchange and gel filtration chromatography. The enzyme was purified to 529.43 fold and showed final specific activity of 280.6 IU/mg with 43.5% yield. SDS-PAGE revealed that the purified enzyme had a molecular weight of 48 kDa. The purified enzyme tested against A375-C6, MCF-7, HCT-113 and Jurkat, clone E6-1cell lines showed I.C.50 value of about 5. 21, 6.3,8 and 3.13 U/ml respectively. ADI obtained from Vibrio alginolyticus 1374 has several beneficial properties needed for its use as a therapeutic and industrial enzyme. It was active over wide range of pH (4-10) and temperature (25°-50°C), had high substrate specificity etc. Moreover it showed broad range of activity against cancer cells lines of human origin. Further animal model studies must be carried out to develop ADI as a potential drug.
Authors and Affiliations
Rahamat Unissa, M. Sudhakar, A. Sunil Kumar Reddy
Keywords
Related Articles
- EP ID EP350816
- DOI 10.9734/BMRJ/2016/23592
- Views 89
- Downloads 0
Antibiotic Susceptibility Test of Klebsiella pneumoniae and K. oxytoca Isolated from Different Clinical Samples and Perform Random Amplified Polymorphic DNA among K. pneumoniae
Aims: To study the prevalence of MDR and ESBL producing Klebsiella pneumoniae and Klebsiella oxytoca and perform RAPD (Random amplified polymorphic DNA) by optimizing PCR among isolated ESBL producing Klebsiella pneumoni...
Mycological Quality of Relief Maize Meal and Nutritional Supplement of Maize Product (Unimix) in Moyale, Northern Kenya
Aims: Determination of mycological quality of relief maize meal and Nutritional supplement of maize product (Unimix) in Moyale, Northern Kenya as regards aflatoxin producing fungi and aflatoxin Contamination. Study Desig...
Cultural Isolation and PCR Based Assay for Detection of flaA gene of Campylobacter jejuni from Acute Diarrheic Patients in Tertiary Care Hospital at Dhaka, Bangladesh
Aim: Among bacterial causes of acute diarrhea Campylobacter species is frequently responsible. Campylobacter spp. is the leading agents of bacterial gastroenteritis in developed as well as developing countries. This stud...
Effects of Ascorbic Acid and Levamisole on the Course of Newcastle Disease in Adult Japanese Quails
Aims: To investigate the effects of ascorbic acid, levamisole and their combination on the course of Newcastle disease in adult Japanese quails. Study Design: Randomized controlled trial. Methodology: The study was condu...
Food-borne Bacteria: Occurrences, Multidrug Resistant Patterns and Susceptibility to Aqueous Leaf Extracts of Acalypha hispida (Linn)
The occurrence and susceptibility of multidrug resistant (MDR) bacteria obtained from food to aqueous leaf extracts of Acalypha hispida (ALEAH) were determined using standard bacteriological and disc diffusion methods. I...