EVALUATION OF IN VITRO ANTICANCER ACTIVITY OF SYMPLOCOS RACEMOSA BARK AGAINST HEPATOCELLULAR CARCINOMA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To investigate in vitro anticancer activity of different extracts of bark of Symplocos racemosa against hepatocellular carcinoma.Methods: Different successive extracts of Symplocos racemosa bark were prepared using hexane, chloroform, ethyl acetate, n-butanol and water and were tested in vitro for cytotoxicity using (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay in rat normal liver cells (BRL-3A) and human hepatocellular carcinoma (Hep3B) cells.Results: Ethyl acetate and chloroform extract of Symplocos racemosa exhibited cytotoxicity against human hepatocellular carcinoma (Hep3B) cells in vitro with IC50 value (µg/ml) of 63.45 and 75.55 respectively and not affected the normal liver (BRL-3A) cells.Conclusion: Symplocos racemosa bark extracts showed potential cytotoxic effects on human hepatocellular carcinoma cells. The anticancer activity exhibited by ethyl acetate and chloroform extract might be due to presence of phenolics and flavonoid constituents present in the bark. Ethyl acetate extract can further be explored for possible cytotoxic activity using in vivo models of liver cancer.Â
Authors and Affiliations
Niyati S. Acharya, Unnati R. Shah, Ripal G. Shah, Sanjeev Acharya, Lal Hingorani
Keywords
Related Articles
- EP ID EP578193
- DOI -
- Views 60
- Downloads 0
IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF MIMOSA PUDICA LINN EXTRACT AND L-MIMOSINE ON LYMPHOMA DAUDI CELLS
Objective: The present study is an attempt to investigate the antioxidant and anticancer potential of hydroalcoholic extract of Mimosa pudica Linn (Mimosaceae) and L-Mimosine on Daudi cell line.Methods: The analysis of t...
EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL PROPERTIES OF CALOTROPIS PROCERA (AIT) R. BR. LEAVES
Objective: The correlation of phytochemical and antibacterial attributes of Calotropis procera leaves with an intent to understand its potential for application in different clinical investigations. Methods: The differen...
DEVELOPMENT AND VALIDATION OF A DRIED BLOOD SPOT LC-MS/MS ASSAY TO QUANTIFY GEMCITABINE IN HUMAN WHOLE BLOOD: A COMPARISION WITH AND WITHOUT CYTIDINE DEAMINASE INHIBITOR
Objective: The purpose of this paper is to develop and validate a LC-MS/MS method for the quantification of gemcitabine in whole human blood using dried blood spots.Methods: Gemcitabine fortified blood samples without te...
ACUTE TOXICITY OF DIFFERENT SIZES OF SILVER NANOPARTICLES INTRAPERITONALLY INJECTED IN BALB/C MICE USING TWO TOXICOLOGICAL METHODS
Objective: This study aimed to evaluate the acute toxicity of intraperitoneally administrated silver nanoparticles (AgNPs) with different particle sizes in BALB/c mice.Methods: Citrate-capped AgNPs were prepared by citra...
ASSESSMENT OF GENETIC DIVERSITY IN TINOSPORA CORDIFOLIA BY INTER SIMPLE SEQUENCE REPEATS (ISSR) AND EXPRESSED SEQUENCE TAGGED- SIMPLE SEQUENCE REPEATS (EST-SSR)
Objective: In this study, assessment of genetic diversity was carried out using two kinds of molecular markers: Inter-Simple Sequence Repeats (ISSR) and Expressed Sequence Tag Simple Sequence Repeats (EST-SSR) in T. cord...