Evaluation of leaf proteome in wheat genotypes under drought stress
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 3
Abstract
Drought stress in plants, the change (increase or decrease) in the production of plant proteins. Proteomics in recent years one of the most powerful tools that help us to study the changes in protein In order to investigate the proteome of wheat leaves in response to terminal drought, two genotypes susceptible and resistant wheat genotypes were evaluated under irrigated (non-stress) and rain-fed (stress) conditions. After applying stress and extraction of leaf proteins, two-dimensional gels were prepared and scanned. Analysis of gel images was performed using Same Spot Progenesis. About 657 protein spots were identified by the software. After alignment of the spots and their correspondence, 148 spot were identified visually and by using the software and statistical analysis was carried out. Five spots with Fold ≥ 1/5 at P ≤ 0/05 were identified, of which 4 spots were significant at P ≤ 0/05 and 1 spot was significant at P ≤ 0/01.
Authors and Affiliations
K. Moradpour| Graduate MSc of Agricultural Biotechnology, Razi University, Kermanshah, Iran, A. Najaphy| Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, S. Mansoorifar| Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, A. Mostafaie| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, M. Bakhtiari| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, S. Kiani| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Keywords
Related Articles
- EP ID EP13093
- DOI -
- Views 298
- Downloads 12
Antimicrobial activity of Trachyspermum ammi essential oil against human bacterial
The aim of this study was to evaluate the antimicrobial activities of Trachyspermum ammi, essential oil against different kinds of microorganisms by microtiter plate method. All 36 isolates of (12 Klebsiella pneumoniae...
Effect of seasons and different substrates on growth and reproduction of the compost worm Eisenia Fetida
The experiment was carried out in a factorial arrangement with the base of randomized complete block design in three repetitions. The first factor consisted of 11 bed for eating worm and the second factor is the differ...
Effect of plant growth regulators on rooting of henna (Lawsonia inermis L.)
Studies have shown that rooting substrate is one of the effective factors at rooting of hard rhizogenetic plant such as Henna. The purpose of this study is to determine an appropriate concentration of Naphthalene Aceti...
A Study on the Potential of Ochratoxin Production by Aspergillus Sp. of Gilan, Mazandaran and Golestan Provinces and Ochrotoxin Rate of Producing Grown Cell Biomass in the Laborating Conditions
Objective: Molds produce poisons called mycotoxins,OTA is one of the most relevant mycotoxins which is generally produced by Aspergillus, its presence in food and feed products being regulated in many countries. Method...
Health Positive Uses of Genetically Modified Food Crops as a Source of Biopharmaceuticals
Molecular farming, or bio-pharming, has recently received much of attention for production of valuable recombinant proteins, with a few already being marketed. The use of whole plants for synthesis of pharmaceutical pr...