Evaluation of leaf proteome in wheat genotypes under drought stress
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 3
Abstract
Drought stress in plants, the change (increase or decrease) in the production of plant proteins. Proteomics in recent years one of the most powerful tools that help us to study the changes in protein In order to investigate the proteome of wheat leaves in response to terminal drought, two genotypes susceptible and resistant wheat genotypes were evaluated under irrigated (non-stress) and rain-fed (stress) conditions. After applying stress and extraction of leaf proteins, two-dimensional gels were prepared and scanned. Analysis of gel images was performed using Same Spot Progenesis. About 657 protein spots were identified by the software. After alignment of the spots and their correspondence, 148 spot were identified visually and by using the software and statistical analysis was carried out. Five spots with Fold ≥ 1/5 at P ≤ 0/05 were identified, of which 4 spots were significant at P ≤ 0/05 and 1 spot was significant at P ≤ 0/01.
Authors and Affiliations
K. Moradpour| Graduate MSc of Agricultural Biotechnology, Razi University, Kermanshah, Iran, A. Najaphy| Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, S. Mansoorifar| Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, A. Mostafaie| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, M. Bakhtiari| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, S. Kiani| Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Keywords
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