Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains
Journal Title: Sultan Qaboos University Medical Journal - Year 2018, Vol 18, Issue 2
Abstract
Objectives: The objectives of this study were to quantitatively estimate the number of mitotic figures (MFs) and evaluate the cellular and nuclear features of various histological grades of oral squamous cell carcinoma (OSCC) using Feulgen and 1% crystal violet stains. Methods: This case-control study took place at the Dr D. Y. Patil Dental College & Hospital in Mumbai, Maharashtra, India, between June and December 2016. A total of 51 samples were retrieved from the hospital archives. Of these, 15 well-differentiated, 15 moderately-differentiated and six poorly-differentiated OSCC samples formed the case group while 15 samples of normal gingival mucosa constituted the control group. Each sample was dyed using Feulgen and 1% crystal violet stains and the mitotic count, nuclear area (NA), cellular area (CA), nuclear perimeter (NP), cellular perimeter (CP) and nuclear-to-cytoplasmic (N/C) ratio was calculated using computeraided morphometry techniques. Results: The number of MFs visible per field was significantly higher in Feulgen-stained sections as compared to those stained with crystal violet (P = 0.050). In addition, the NA, NP, CA and CP values and N/C ratios of samples in the experimental group increased significantly in accordance with an increase in OSCC grade (P <0.001). Conclusion: The Feulgen stain is more reliable than 1% crystal violet in terms of the selective staining of MFs. Moreover, the findings of this study indicate that computer-based morphometric analysis is an effective tool for differentiating between various grades of OSCC.
Authors and Affiliations
Kashmira Kesarkar, Avinash Tamgadge, Treville Peirera, Sandhya Tamgadge, Swati Gotmare, Pooja Kamat
Keywords
Related Articles
- EP ID EP34689
- DOI http://dx.doi.org/10.18295/squmj.2018.18.02.005
- Views 338
- Downloads 0
To Be or Not To Be Exposed to Direct Sunlight Vitamin D Defciency in Oman
Only small amounts of vitamin D come from dietary sources as it is mainly synthesised in the skin from the ultraviolet B (UVB) fraction of sunlight if the person is sufciently exposed to direct sunlight. Vitamin D defc...
Congenital Fusion of the Trapezium and Trapezoid
Clinical Signifcance of an Unusual Variation Anomalous additional belly of the sternothyroid muscle
Te infrahyoid muscles are involved in vocalisation and swallowing; among these, the sternothyroid muscle is derived from the common primitive sheet. Te improper differentiation of this muscle may therefore result in m...
Ranolazine A true pluripotent cardiovascular drug or jack of all trades, master of none?
Cardiovascular disease (CVD) is a leading cause of morbidity and mortality worldwide. Although the majority of patients with CVD are treated with interventional procedures, a substantial number require medical therapy...
Medical Education Units History, Functions, and Organisation
Most medical schools have established a medical education unit (MEU) or similar bodies in response to various reforms in medical education. Such units have a variety of titles and operate either independently or under t...