Evaluation of MPT 64 Antigen for Early Differentiation Between Mycobacterium Tuberculosis Complex And Nontuberculous Mycobacteria in Smear Negative Patients
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 10
Abstract
Tuberculosis (TB) is a global pandemic & India is one of the major TB endemic countries. The worldwide magnitude of modern tuberculosis is so great that in April 1993 the World Health Organization (WHO) declared tuberculosis to be a global emergency.It is important to accurately characterize Mycobacterium tuberculosis complex (MTBC) and Nontuberculous Mycobacteria (NTM) as NTM are inherently resistant to many conventional anti-tuberculosis drugs, require modified treatment regimens and are often misdiagnosed as multidrug-resistant tuberculosis (MDR). Differentiation between MTBC and NTM is done by either conventional biochemical tests or molecular methods. The former are time consuming and cumbersome while the latter are expensive, require trained personnel and special laboratory setup. Hence there is need for a rapid, accurate and simple test for characterization of Mycobacteria. In this study we evaluate the MPT64 antigen kit for early differentiation between MTBC and NTM in smear negative but culture positive patients. The study period was between Januaryto December’2014. We included 200smear-negative but clinically suspected pulmonary and extra-pulmonary tuberculosis patientswhoattended or gotadmitted to the Chest Medicinedepartment of Bankura Sammilani Medical College. The samples were cultured in L-J media or BacT/Alert 3D system. The positive culture was tested by conventional biochemical test and with MPT64 kit to detect MTBC& differentiate MTBC from NTM.15% samples were culture positive among 200 patients. 18 (9%)MTBC and 12 (6%) NTM were detected by conventional system and same result was found by MPT-64 system but with much rapidity. So MPT 64 Antigen detection kit may be a good alternative to biochemical and molecular methods of identification of Mycobacterial species.
Authors and Affiliations
Rehena Sarkar, Debalina Das, JayantaBikash Dey, Abhijit Mandal, Soumick Sarkar, Koushik Ghosh
Keywords
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- EP ID EP244359
- DOI 10.9790/0853-1610089093
- Views 76
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