Evaluation of Mucoadhesive PLGA Microparticles for Nasal Immunization
Journal Title: The AAPS Journal - Year 2010, Vol 12, Issue 2
Abstract
In this study, hepatitis B surface antigen (HBsAg) loaded poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared and coated with chitosan and trimethyl chitosan (TMC) to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain PLGA microparticles demonstrated negative zeta potential. However, coated microparticles showed higher positive zeta potential. Results indicated that TMC microparticles demonstrated substantially higher mucin adsorption when compared to chitosan-coated microparticles and plain PLGA microparticles. The coated and uncoated microparticles showed deposition in nasal-associated lymphoid tissue under fluorescence microscopy. The coated and uncoated microparticles were then administered intranasally to mice. Immune-adjuvant effect was determined on the basis of specific antibody titer observed in serum and secretions using enzyme-linked immunosorbent assay. It was observed that coated particles showed a markedly increased anti-HBsAg titer as compared to plain PLGA microparticles, but the results were more pronounced with the TMC-coated PLGA microparticles.
Authors and Affiliations
Dilip Pawar, Amit K. Goyal, Sharad Mangal, Neeraj Mishra, Bhuvaneshwar Vaidya, Shailja Tiwari, Arvind K. Jain, Suresh P. Vyas
Keywords
Related Articles
- EP ID EP681367
- DOI 10.1208/s12248-009-9169-1
- Views 67
- Downloads 0
Stereoselective pharmacokinetics of ifosfamide in male and female rats
The stereoselective pharmacokinetics of ifosfamide (IF) were investigated in male and female Sprague-Dawley rats. Following intravenous administration of IF deuterium-labeled pseudoracemates into rats at 40 mg/kg, IF ena...
Performance of Methods for Handling Missing Categorical Covariate Data in Population Pharmacokinetic Analyses
The online version of this article (doi:10.1208/s12248-012-9373-2) contains supplementary material, which is available to authorized users.
Resistance to the Translation Initiation Inhibitor Silvestrol is Mediated by ABCB1/P-Glycoprotein Overexpression in Acute Lymphoblastic Leukemia Cells
Protein synthesis is a powerful therapeutic target in leukemias and other cancers, but few pharmacologically viable agents are available that affect this process directly. The plant-derived agent silvestrol specifically...
Quantitative Pharmacology Approach in Alzheimer’s Disease: Efficacy Modeling of Early Clinical Data to Predict Clinical Outcome of Tesofensine
Effective therapeutic options for Alzheimer’s disease (AD) are limited and much research is currently ongoing. The high attrition rate in drug development is a critical issue. Here, the quantitative pharmacology...
An interactive algorithm for the assessment of cumulative cortisol suppression during inhaled corticosteroid therapy
The objective of the study was to develop an algorithm based on a pharmacokinetic-pharmacodynamic (PK/PD) modeling approach to quantify and predict cumulative cortisol suppression (CCS) as a surrogate marker for the syst...