Evaluation of Optimum Condition for Reducing Sugar Production from Eichhornia Crassipes Biomass by Saccharification through Trichoderma Reesei
Journal Title: International Journal of Botany and Research (IJBR) - Year 2017, Vol 7, Issue 5
Abstract
Water Hyacinth is used as renewable energy raw material for sugar production and then fuel production because, its availability is easy and rate of propagation is very high. It is an aquatic weed, tolerates variation in pH and temperature and is unaffected by toxic substances. Determination of reducing sugar from lignocellulosic biomass of Water Hyacinth was carried out by saccharification process, using micro fungi as trichoderma reesei. It was achieved using dinitro salicyclic assays (DNS method). Reducing sugar contents were evaluated maximum at optimum period. The amount of sugar contents were maximum which were 547 μg/ml in untreated condition and 596 μg/ml in treated condition at optimum inoculation period these were found after inoculation of seventh day and 365 μg/ml, 458 μg/ml and373 μg/ml in untreated condition and 521 μg/ml, 593 μg/ml and 492 μg/ml in treated condition at optimum conditions of pH, temperature and substrate concentration which were 5 or 5.5, 300C and 2.0 or 2.5 ml respectively. Here the two types of samples were prepared. One was treated with 1%H2SO4 and other was untreated. The reducing sugar contents were obtained more in treated condition in compression to untreated condition. The present study evaluated Water Hyacinth as raw material for possible strategies by conversion of hydrolysate to reducing sugar was maximized. For this the effect of optimum condition such as inoculation period, pH, temperature and substrate concentration on conversion of Water Hyacinth biomass to reducing sugar was studied.
Authors and Affiliations
Tirthesh Kumar Sharma, Ramendra Singh
Keywords
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