Evaluation of phytochemicals and antibacterial activity of leaf and leaf derived callus extracts of Artemisia annua L. and Sauropus androgynus (L.) Merr.
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 4
Abstract
The present study focuses on the use of salicylic acid as a plant growth hormone for the induction of callus in Sauropus androgynus and Artemisia annua plants. Higher induction of callus of 72% in S. androgynus (NAA+BAP+SA at +2.5+2.5 mg/ l concentration) and 64% in A. annua (NAA+Kn+SA at 1.0+2.5+2.5 mg/ l concentration) were observed when compared to 52% and 51%, respectively in individual supplementation of plant growth regulators. The phytochemical analysis also revealed the presence of maximum phytochemicals in callus extract compared to leaf extracts of S. androgynus and A. annua there by corroborating with the results of callus induction. The methanol extracts of both callus and leaf extracts of S. androgynus and A. annua exhibited antibacterial activity against all the test pathogens viz., S. aureus, B. subtilis, S. typhi and E. coli. A maximum inhibition zone of 20 mm was observed against S. typhi in methanol callus extract of S. androgynus followed by 18 mm inhibition zone against S. aureus with same extract. It was evident from the results both callus and leaf extracts of the selected plants possessed potential antibacterial activity against all the test pathogens with one or the other solvent extracts. The findings provide a new facelift for callus induction using salicylic acid in selected plants and also pave way for identification of novel compounds for drug development from S. androgynus and A. annua.
Authors and Affiliations
Manasa Govindaraju, Mahendra Chikkamadaiah, Murali Mahadevamurthy, Mahesh Holenarsipura Mylari, Sudarshana Mysore Shankar Singh
Keywords
Related Articles
- EP ID EP286062
- DOI -
- Views 75
- Downloads 0
Studies of alternaria black spot disease of pomegranate caused by Alternaria alternata in Punjab
Alternaria black spot of pomegranate caused by Alternaria alternata pose significant economic losses in India as it reduce the crop yield. Farm survey was undertaken at Punjab Agricultural University, Pomegranate Researc...
Mapping of coconut growing areas in Tamil Nadu, India using remote sensing and GIS
Importance of remotely sensed data for inventorying, mapping, monitoring and for the management and development planning for the optimum utilization of natural resources has been well established. Though, a lot of applic...
Effect of dates of sowing and varieties on yield and quality of cluster bean (Cyamopsistetra gonoloba L.)
A field experiment was conducted at Agricultural College Farm, Bapatla (Andhra Pradesh), study the effect of different sowing dates on the yield and quality of different varieties of clusterbean. The experiment was laid...
Root architecture and rhizobial inoculation in relation to drought stress response in common bean (Phaseolus vulgaris l.)
The present study was aimed at assessing the root traits and rhizobial inoculation in relation to drought in common bean, Phaseolus vulgaris. Drought caused the largest decrease in shoot biomass followed by plant height,...
Response surface optimization for development of Dragon fruit based ready to serve drink
Dragon fruit based ready to serve drink (RTS) was formulated using dragon fruit (60-80% v/v), grape juice (0-10% v/v) and sugar syrup (2-6% v/v). The juice concentrations were optimized using response surface methodology...