Evaluation of purine metabolites - creatinine index to predict the rumen microbial protein synthesis from urinary spot samples in Barbari goats
Journal Title: Journal of Animal and Feed Sciences - Year 2011, Vol 20, Issue 4
Abstract
an alternative to performing a total urine collection to predict the microbial nitrogen (MN) supply in goats. The study was conducted in two phases. Phase I was comprised of fasting metabolism trial of eight goat bucks (19.70±0.59 kg mean body weight) to quantify the contribution of basal PM excretion. PM excretion decreased (P<0.05) with feed restriction and the mean value (µmol/ kgW0.75/d) observed during fasting was 250.09±5.11. Phase II examined the response of PM excretion to feed intake and evaluated the usefulness of purine metabolites: creatinine (PMC) index in spot urine samples for prediction of rumen MN supply. The concentration of PM in total urine and spot urine was quantified in eight goat bucks (20.24±0.74 kg mean body weight) allocated in two sets of 4×4 Latin square design at four fixed levels of feed intake (95, 80, 60 and 40% of voluntary feed intake) on diet based on wheat straw and concentrate mixture (50:50). Total PM excretion increased linearly with level of intake. Urinary excretion of creatinine did not differ among groups, while the PMC index calculated from total urine and spot urine increased (P<0.05) with level of feed intake. PMC index of spot urine differentiated the feeding levels at 6 and 12 h post feeding. The relationship between PMC index in the spot urine samples collected 12 h after the morning feed and the daily total PM excretion was the highest (R2 =0.82). The results confirm that PMC index in spot urine samples can serve as a potential tool for the quantification of MN supply in goats.
Authors and Affiliations
S. K. George, A. K. Verma, U. R. Mehra, M. T. Dipu, P. Singh
Keywords
Related Articles
- EP ID EP75049
- DOI -
- Views 118
- Downloads 0
Estimation of substrate-energetic fluxes in lactating cows
In this article an algorithm estimating the basic metabolic fluxes in lactating cows is described that uses data of substrates absorption, heat production, respiratory quotient and milk productivity. The flux diagram per...
Evaluation of chemical composition, in situ degradability and in vitro gas production of ensiled and sun-dried mulberry pomace
The study was conducted to evaluate the nutritive value of mulberry pomace (MP) treated with sun-drying (SD), ensiling (EN), and ensiling with a Lactobacillus additive (ELA), respectively. The chemical composition, in si...
Standardized ileal digestibility of amino acids in some cereals, rapeseed products and maize DDGS for broiler chickens at the age of 14 days
The experiment was conducted to assess the ileal digestibility values for the purpose of evaluating the ability of 14-day-old broilers to utilize the amino acids (AA) from different plant feeds. The feeds included two ce...
The nutritional value of narrow-leafed lupin (Lupinus angustifolius) for broilers
The nutritional value of four cultivars of Lupinus angustifolius L. seeds was investigated. The experiment was conducted on 50 male 16-day-old Ross 308 chickens. The birds were randomly allocated to five dietary treatmen...
Effects of dietary fish oil on NFκ B gene expression and related signaling in spleen of chickens stimulated with lipopolysaccharide
The study was conducted to investigate the effects of fish oil and maize oil on nuclear factor kappa B (NFκB) gene expression and the downstream pathways of intracellular signaling in spleen of chickens after lipopolysac...