Evaluation of Resistance Pattern and Plasmid Profile of Staphylococcus Species Isolated from Clinical and Community Samples in Ibadan South-West, Nigeria
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 3, Issue 3
Abstract
Aims: Staphylococcus species have been a major human pathogen of public health importance globally. This study was designed to evaluate the resistance pattern and plasmid profile of Staphylococcus species isolated from clinical and community settings. Methodology: Staphylococcus species from clinical (55) and community (53) which were previously isolated in University of Ibadan and her teaching hospital and identified as S. epidermidis (92.6%), S. aureus (6.5%) and S. xylosus (0.9%) were used. The antibiogram and plasmid profiles were determined by standard procedures. Results: In clinical isolates of S. epidermidis, 30.9, 34.5, 40.0, 41.8, 60.0, 76.4, and 89.1% were resistant to chloramphenicol (CHL), streptomycin (STR), erythromycin (ERY), gentamycin (GEN), tetracycline (TET), cotrimoxazole (COT), and cloxacillin (CXC) respectively. Correspondingly, in community isolates of S. epidermidis, 28.3, 32.1, 50.9, 26.4, 58.5, 90.6 and 92.5% were resistant to these antibiotics. The only clinical S. xylosus isolated was resistant to all the antibiotics except CHL and STR. In the clinical isolates of S. aureus, 5.5, 5.5, 7.3, 7.3, 7.3, 9.1 and 9.1% were resistant to ERY, CHL, STR, GEN, TET, COT and CXC respectively. In community isolates, only one S. aureus was resistant to COT, CHL, ERY, GEN and STR while two were resistant to CXC. Plasmid profiling showed that 33/35 (94.3%) of clinical and 17/19 (89.5%) of community isolates had plasmid of size 23.13 kb. Conclusion: The increasing resistance and similarity of plasmid profile of the community isolates to clinical isolates call for urgent establishment of antibiotic surveillance system to minimize the emergence of drug resistance pathogens in the community.
Authors and Affiliations
Cajethan O. Ezeamagu, Obasola E. Fagade, Stella I. Smith, Adeniyi A. Ogunjobi
pH Effect and pH Changes during Biocellulose Production by Gluconacetobacter xylinus in Moringa oleifera Tea-Sugar Medium
The study was carried out to determine the effect of pH and pH changes accompanying Biocellulose (BC) production by Gluconacetobacter xylinus (GX) in a Moringa oleifera tea-sugar medium. The authors adopted experimental...
An Effective Cloning, Heterologous Expression and Physiological Activity in Lactococcus lactis NZ9000 of Catalase Gene from Escherichia coli DH5α
In order to clone active Catalase gene from bacteria, we introduced a method of shotgun integrating specific screen from Escherichia coli DH 5. Genome DNA was extracted from E. coli DH5 and partially digested with Sau3A...
Molecular Characterization of ESBLs Genes among Multi-drug Resistant Klebsiella species in Ile-Ife South-West, Nigeria
Aim: This study was carried out to determine the presence of ESBLs genes (TEM, CTX-M, SHV, AAC and OXA types of Beta-lactamases) in clinical isolates of Klebsiella species producing extended spectrum beta-lactamase. Ext...
Immunomodulatory and Toxicological Effect of Goya Extra Virgin Olive Oil in Albino Rat Orogastrically Dosed with Salmonella typhi
The immunomodulatory and toxicological properties of Goya extra virgin olive oil on albino rats orogastrically dosed with Salmonella typhi were evaluated in this study. Twenty albino rats were randomly distributed into f...
Bio Diversiity and Cultivation Parameters, Mycochemical Analysis of Wild Mushrooms Collected from Western Ghats of Tamilnadu, India
Western ghats of Tamilnadu contains varieties of wild mushrooms. Macroscopic fungi were collected from Western Ghats of Tirunelveli District, especially Kalakad Mundanthurai Tiger Reserve & Range in Tamilnadu to document...