EVALUATION OF SAFETY PROFILE AND CEREBROPROTECTIVE POTENTIAL OF DIMETHYL FUMARATE (DMF) AGAINST ISCHEMIA AND REPERFUSION INDUCED CEREBRAL INJURY IN WISTAR RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 2
Abstract
Objective: Dimethyl fumarate (DMF) is the methyl ester of fumaric acid and used in the treatment of psoriasis, multiple sclerosis, etc. The present study was carried out to reveal safety as well as to find out the cerebro-protective potential of DMF against ischemia and reperfusion-induced cerebral injury in Wistar rats.Methods: Acute and sub-acute toxicity tests were performed in mice at various increasing doses of DMF as per OECD guidelines 420 and 407 respectively. The cerebral ischemia-reperfusion injury was induced by bilateral common carotid artery (BCCA) occlusion for 1 h and reperfusion for 5 h in Wistar rats. The DMF was administered orally at doses 10 mg/kg and 20 mg/kg for the assessment of its cerebro-protective potential by evaluating % infarction and % protection of cerebral tissue using TTC staining method.Results: The present study revealed that DMF is a safe drug as mortality was not observed in acute toxicity study up to 2000 mg/kg on oral administration, at the same time in sub-acute toxicity test DMF has showed no alteration in various parameters except a decrease in lymphocyte count. The DMF has shown a reduction in the % infarction. DMF at doses 10 mg/kg and 20 mg/kg has imparted 27.96±1.55% and 58.98±2.3% protection against ischemia and reperfusion-induced cerebral injury in Wistar rats.Conclusion: The DMF is the relatively safe drug with significant cerebro-protective potential.
Authors and Affiliations
Ketan Vinayakrao Hatware, Annapurna Akula
Keywords
Related Articles
- EP ID EP575849
- DOI 10.22159/ijpps.2017v9i2.16098
- Views 63
- Downloads 0
ISOLATION AND ANTIDIABETIC ACTIVITY OF NEW LANOSTENOIDS FROM THE LEAVES OF PSIDIUM GUAJAVA L.
Objective: Diabetes mellitus is a chronic metabolic disease which affects our body's ability to use the energy found in food. Our study was planned to isolate chemical constituents from the leaves of Psidium guajava L. (...
UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF SOLIFENACIN SUCCINATE IN TABLETS
Objective: To develop a simple and cheap UV spectrophotometric method for the quantitative estimation of Solifenacin succinate (5mg) in tablets and validate as per ICH guidelines.Methods: The optimized method uses a solv...
EFFECTS OF CIPROFLOXACIN IN COMBINATION WITH EITHER AMINOGUANIDINE OR MECLOFENAMIC ACID IN MODULATING S. AUREUS INDUCED SEPTIC ARTHRITIS IN MICE
Objective: Staphylococcus aureus (S. aureus) is a potent causative organism of septic arthritis. In this study, ciprofloxacin was used to nullify the bacterial burden in mice infected with pathogenic strain of S. aureus....
N-PHENYLBENZAMIDE SYNTHESIS BY NUCLEOPHILIC SUBSTITUTION WITH 1,3-DIPHENYLTHIOUREA
Objective: N-phenylbenzamides are important and biologically active compounds. N-phenylbenzamides have been synthesized by some routes. An attempt has been made to find out the new route to synthesize N-phenylbenzamides....
ANTIDIABETIC ACTIVITY OF ASYSTASIA GANGETICA (L.) T. ANDERSON FLOWER EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Objective: Asystasia gangetica has long been used in the antiasthmatic medicinal preparations of traditional healers Nigeria of South Africa, in India the plant is being used for treating diabetes and microbial infection...