EVALUATION OF SURFACE CONTAMINATION OF BACTERIA IN VARIOUS DENTAL CLINICS WITH SPECIAL REFERENCE TO OBLIGATE AND FACULTATIVE ANAEROBIC SPORE BEARING BACILLI
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2014, Vol 3, Issue 3
Abstract
Introduction: The occupational health and safety is an important prerequisite in dental clinic setup for well being of both the doctor and patient. Both the patient and dentist are always at the risk of infections. Aim and objectives: There is no proper literature on the survey of bacterial spores, especially of Clostridium species in dental clinics. Hence an attempt has been made in the present pilot study to evaluate the surface contamination with special reference to bacterial spores. Materials and methods: Various dental clinics from Chennai city, India were selected for the present study. Samples were collected from two clinics each from endodontic, prosthodontic, orthodontic, and periodontic. In each clinic important places were selected for sampling. The samples were collected in the form of swabs. The swabs thus obtained were inoculated into Robertson Cooked Meat Medium and was incubated in anaerobic condition at 370C for 7 days. Each day the tubes were examined for turbidity and colour change and were noted. At the end of 7th day the smear was prepared from each tube and gram staining was performed. The gram stained slides were examined microscopically for the presence of spore bearing bacilli especially with special reference to terminal spore bearing bacilli. Results and conclusion: From the present study it is clear that the dental clinics invariably posses a lot of aerobic and anaerobic spores irrespective of stringent disinfection procedures. Hence it is mandatory for the dental clinics to undergo periodical microbiological surveillance and to take proper steps in the control of bacterial spores.
Authors and Affiliations
Kannan I| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India, Corresponding author email:kannan_iyan@hotmail.com, Jessica Yolanda Jeevitha| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India, Sambandam Cecilia| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India, Jayalakshmi M| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India, Premavathy RK| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India, Shantha S| Department of Microbiology, Tagore Dental College and Hospital, Rathinamangalam, Chennai, Tamil Nadu, India
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- EP ID EP11268
- DOI 10.5958/2319-5886.2014.00395.6
- Views 257
- Downloads 14
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