EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chamber (DSFC) and Tube formation assay.Methods: Three different concentrations of drug solution (40µM, 80 µM, 120 µM) and (50 µM, 100 µM, 150 µM) were applied topically during CAM and DSFC assay. These assays were performed to detect antiangiogenic effect of selected molecules. In tube formation assay, Human Umbilical Vein Endothelial Cells (HUVECs) were treated with various concentrations.Results: Antiangiogenic results showed that different concentration of taxifolin and phloretin inhibited new blood vessels and vessels branches per area of CAMs. Similarly, there are reduced numbers and diameter of blood vessels when the doses of the drug increased which are observed in different experimental groups in DSFC model. In vitro anti angiogenic effect of our molecules was further confirmed by inhibition of tube formation on matrigel matrix that involved HUVECs. All the test groups were compared with the control group.Conclusion: The data reported here provide evidence that our flavonoids taxifolin and phloretin revealed prominent anti angiogenic activities.Â
Authors and Affiliations
Md Wasimul Haque, Shakti Prasad Pattanayak, Barij Nyan Sinha
Keywords
Related Articles
- EP ID EP578130
- DOI -
- Views 134
- Downloads 0
EFFECT OF HEATING TIME AND HEAT ON THE PHYSICOCHEMICAL AND ANTIOXIDATIVE PROPERTY OF FISH (LABEO ROHITA) SKIN OIL
Objective: Determination of oxidative and thermal stability of Labeo rohita skin oil. Methods: Labeo rohita skin oil was extracted by soxhlet method using n-hexane as solvent. Acid value, Free Fatty Acid content, the Pe...
PHYTOCHEMICAL PROFILING OF SUCCESSIVE EXTRACTS OF FRUIT AND STEM BARK OF SOLANUM PUBESCENS
Objective: Solanum (L) is the most representative genus of Solanaceae, Solanum species are a rich source of bioactive compounds which are known to possess a variety of biological activities. Solanum pubescens is a wild p...
VALIDATED SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF ENALAPRIL MALEATE IN PURE AND DOSAGE FORMS
Objective: Simple, sensitive, precise, reproducible and validated visible spectrophotometric methods have been developed for the determination of an angiotensin converting enzyme inhibitor (ACE) drug, namely enalapril ma...
DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC ASSAY METHOD FOR AZACITIDINE AND ITS BULK DRUG
Objective: A novel gradient reverse phase High Performance Liquid Chromatographic (HPLC) method was developed for the quantification of Azacitidine impurities and degradation products in the Azacitidine tablets.Methods:...
GENERATION DEPENDENT TARGETING POTENTIAL OF DONEPEZIL LOADED POLY (PROPYLENEIMINE) DENDRIMER THROUGH GOAT NASAL MUCOSA
Objective: In the domain of nano drug delivery, dendrimers are the most explored bioactive polymeric carrier system. The present work was aimed to study the diffusion potential of different generations of Poly (propylene...