EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chamber (DSFC) and Tube formation assay.Methods: Three different concentrations of drug solution (40µM, 80 µM, 120 µM) and (50 µM, 100 µM, 150 µM) were applied topically during CAM and DSFC assay. These assays were performed to detect antiangiogenic effect of selected molecules. In tube formation assay, Human Umbilical Vein Endothelial Cells (HUVECs) were treated with various concentrations.Results: Antiangiogenic results showed that different concentration of taxifolin and phloretin inhibited new blood vessels and vessels branches per area of CAMs. Similarly, there are reduced numbers and diameter of blood vessels when the doses of the drug increased which are observed in different experimental groups in DSFC model. In vitro anti angiogenic effect of our molecules was further confirmed by inhibition of tube formation on matrigel matrix that involved HUVECs. All the test groups were compared with the control group.Conclusion: The data reported here provide evidence that our flavonoids taxifolin and phloretin revealed prominent anti angiogenic activities.Â
Authors and Affiliations
Md Wasimul Haque, Shakti Prasad Pattanayak, Barij Nyan Sinha
Keywords
Related Articles
- EP ID EP578130
- DOI -
- Views 155
- Downloads 0
ANTICANCER AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF HIPPOPHAE SALICIFOLIA IN EAC INDUCED SWISS ALBINO MICE
Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice.Methods: In vitro cytotoxicity assay has been eva...
SYNTHESIS AND EVALUATION OF SOME BENZOTHIAZOLE DERIVATIVES AS ANTIDIABETIC AGENTS
Objective: The objective of the present research investigation involves synthesis and biological evaluation of antidiabetic activity of benzothiazole derivatives.Methods: A novel series of benzothiazole derivatives 7(a-l...
EFFECT OF CAFFEINE IN EXPERIMENTAL MODEL OF RHEUMATOID ARTHRITIS IN RATS
Objective: Primary objective of this study was to evaluate anti-inflammatory effect of caffeine in complete Freund’s adjuvant model of rheumatoid arthritis. Secondary objective was to compare the topical anti-inflammat...
DRUG RESISTANCE PATTERNS OF CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS IN TERTIARY CARE CENTER OF SOUTH INDIA
Objectives: Staphylococcus aureus were initially described in 1961 and emerged in the last decade as one of the most important nosocomial pathogens. The current study was undertaken to provide data for empirical selecti...
THE EFFECT OF PROPHYLACTIC INHIBITION OF INDUCIBLE NITRIC OXIDE SYNTHASE BY AMINOGUANIDINEON SERUM LEVELS OF SOME ADIPOCYTOKINES IN PRISTANE-INDUCED ARTHRITISIN RATS
Objective: The aim of the present study was to evaluate the effect of prophylactic inhibition of inducible nitric oxide synthase (iNOS) by aminoguanidine (AG) on serum levels of some adipocytokines in pristane-induced ar...