Evaluation of the effect of CaCl2 and alginate concentrations and hardening time on the characteristics of Lactobacillus acidophilus loaded alginate beads using response surface analysis
Journal Title: Advanced Pharmaceutical Bulletin - Year 2012, Vol 2, Issue 1
Abstract
Purpose: This article describes preparation and characterization of beads of alginate containing probiotic bacteria of Lactobacillus acidophilus DMSZ20079. Methods: Fourteen formulations using different alginate (ALG) and CaCl2 concentrations as well as hardening times were prepared using extrusion technique. The prepared beads were characterized in terms of size, morphology, encapsulation efficiency and bacterial viabilities in acid (pH 1.8, 2 hours) condition. Results: The results showed that spherical beads with narrow size distribution ranging from 1.32±0.04 to 1.70±0.07 mm were achieved with encapsulation efficiency higher than 98%. Surface response analysis revealed that alginate concentration was the important factor for the size, shape and encapsulation efficiency of prepared beads. Furthermore, survived bacteria after acid exposure in all prepared beads (63-83%) were significantly higher than those of untreated cells (39%) and enhanced by increasing alginate concentration. Surface response analysis revealed that the effect of all three factors of alginate and CaCl2 concentrations as well as hardening times were significant in acid viability, however alginate concentration played the most important role according to its regression coefficient. Conclusion: Among alginate and CaCl2 concentrations as well as hardening times, alginate concentration was the most variable in the characteristics of Alginate beads.
Authors and Affiliations
Farzaneh Lotfipour, Shahla Mirzaeei, Maryam Maghsoodi
Development and Validation of an HPLC Method for the Analysis of Sirolimus in Drug Products
Purpose: The aim of this study was to develop a simple, rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method for quantification of sirolimus (SRL) in pharmaceutical dosage forms. Meth...
Polymer Percolation Threshold in Multi-Component HPMC Matrices Tablets
Introduction:The percolation theory studies the critical points or percolation thresholds of the system, where one component of the system undergoes a geometrical phase transition, starting to connect the whole system.Th...
Hypoglycemic Activity of Fumaria parviflora in Streptozotocin-Induced Diabetic Rats
Purpose: Fumaria parviflora Lam (Fumariaceae) has been used in traditional medicine in the treatment of several diseases such as diabetes. The present work was designed to evaluate the hypoglycaemic effects of methanolic...
On the mechanism of agglomeration in suspension
Purpose: Agglomeration in suspension is a size enlargement method that facilitates operation of solid processing and preserves the solubilization properties of fine particles. A small quantity of binder liquid is added i...
Hollow microspheres for gastroretentive floating- pulsatile drug delivery: preparation and in vitro evaluation
Purpose: A multiparticular floating-pulsatile drug delivery system was developed for time and site specific drug release of piroxicam. A blend of floating and pulsatile principles of drug delivery system would have the a...