Evaluation of the Effect of Storage on Sterilization and Structural Integrity of Enamel of Teeth Preserved In A Tooth Bank– An In-Vitro Study
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 1
Abstract
Objective: The aim of present study was to evaluate the effect of storage on sterilization and structural integrity of enamel of extracted teeth preserved in a Tooth Tissue Bank. Study design: Forty five freshly extracted teeth were collected and sterilized by ultrasonic waves and 6% H2O2 combination. The teeth were divided into three groups (A ,B, and C) with 15 teeth in each group and were stored in Hank’s Balanced Salts Solution at 4⁰C.The sample teeth of group A,B and C were used to measure the effect of storage on sterilization, enamel hardness and enamel calcium solubility respectively at 3,6, 12 and 18 month intervals of time. Results: More than 50% samples of Group A showed presence of aerobic bacteria after 9 months of storage period. Enamel hardness was not significantly effected up till 3 months of storage period. Enamel solubility increased successively upon storage but increase was not significant till 3 months storage. Conclusion: Teeth stored in a tooth tissue bank maintain their Structural integrity upto 3 months and remain sterile not beyond 6 months time period.
Authors and Affiliations
Manpreet Kaur
Keywords
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