Evaluation of the efficacy of topical ofloxacin, pefloxacin, lomefloxacin, sparfloxacin in the experimental model of corneal ulcer
Journal Title: Journal of Indira Gandhi Institute of Medical Sciences - Year 2018, Vol 4, Issue 1
Abstract
Abstract Objective: This study was designed to evaluate the efficacy of topical ofloxacin, pefloxacin, lomefloxacin, sparfloxacin in the experimental model of corneal ulcer. Methods: Twenty New Zealand albino rabbits of either sex weighing 1.5-2 kg body weight were used for the corneal ulcer efficacy study, Staphylococcus aureus was cultured in 1% peptone water by incubating it at 37̊ c for 48 hrs. The colony forming unit (CFU) was estimated by using standard McFarland’s tube. Sterile Saline (0.05 ml) containing Staph. Aureus 5x 106 CFU per ml was used for the induction of corneal ulcer. About 10-15µl of the incoculum was injected intrastromally into the peripheral cornea of rabbits using a 1 ml tuberculin syringe fitted with 30G needle. After delivery of inoculums, the epithelial layer was carefully removed by scratching with scalpel blade just above the inoculums delivery site. Before induction of corneal ulcer, all the rabbits were anaesthetized. The antimicrobial therapy was initiated after 18 hrs of the inoculation. 50 µl of formulation were instilled four times a day. Treatment was given up to 4 weeks in each group. Results: After 14 days of treatment with lomefloxacin and sparfloxacin a significant reduction in ulcer size was recorded in the animals. Where as the same was achieved in 7 days after topical ofloxacin treatment. Based on this observation, the efficacy of the tested formulations can be graded in this following order i.e pefloxacin = nanoparticulated sparfloxacin>sparfloxacin>lomefloxacin>ofloxacin. While comparing the percentage healing that occurred after two weeks of therapy, (using the ulcer size) pefloxacin (0.3%) showed 100% healing. Conclusion: Efficacy wise pefloxacin was found to be superior as compared to other fluoroquinolones studied.
Authors and Affiliations
Alok Kumar Ravi, Nihar Ranjan Biswas, Thirumurthy Velepandian, Raju Sampangi, Sat Pal Garg, Supriyo Ghose
Keywords
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