Evaluation of the Epigenetic Biomarker Bone Morphogenic Protein 3 for Colorectal Cancer Diagnosis
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 11
Abstract
ABSTRACT Introduction: Colorectal Cancer (CRC) is a common type of cancer with a rising prevalence worldwide. Morbidity and mortality of CRC can be reduced by screening programs and early diagnosis. Currently used screening tests include fecal-based methods are non-invasive and inexpensive, however the sensitivity and specificity of these tests are not high enough. Aim: To evaluat hypermethylation of Bone Morphogenic Protein 3 (BMP3) as a biomarker in early diagnosis of CRC. Materials and Methods: A total of 96 individuals were enrolled in the present case-control study (59 CRC patients versus 37 healthy controls) and Methylation-Specific Polymerase Chain Reaction (PCR) was used to evaluate methylation status of the BMP3 gene in plasma samples and colonoscopy tissue biopsies. Results: In plasma samples, 75% of CRC patients showed hypermethylation in the BMP3 gene versus only 30% of the controls. In colonoscopy tissue biopsies, BMP3 showed hypermethylation in 81.6% of CRC patients. Specificity and sensitivity of this gene in CRC diagnosis were found to be 76% and 66% respectively. Conclusion: The results of the present study showed that BMP3 in combination with other genes could be used as a non-invasive and promising biomarker in screening and diagnosis of CRC.
Authors and Affiliations
Hasan Ashoori, Mohammad Ebrahim Ghamarchehreh, Mahmood Tavallaei, Shahla Mohammad Ganji, Mostastafa Hosseini, Mohammad Zolfaghari, Zahra Ghamarchehreh, Farnaz Vahidian
Keywords
Related Articles
- EP ID EP524677
- DOI 10.7860/JCDR/2018/37101.12260
- Views 81
- Downloads 0
Histomorphological Lung Changes and Cause of Death Correlation: An Autopsy Based Study in a Tertiary Care Centre
ABSTRACT Introduction: Organ-specific pathological changes that are seen during autopsy give a clearer picture of the cause of death and help to correlate with the clinical scenario. Aim: To study the histomorphological...
Central Variant of Posterior Reversible Encephalopathy Syndrome – A Rare Case Report
Posterior Reversible Encephalopathy Syndrome (PRES) is a clinicoradiological condition associated with headache, altered mental status, dizziness and white matter vasogenic oedema in parieto-occipital region. It is often...
Primary Multidrug-Resistant Extrapulmonary Tuberculosis Presenting as Empyema Necessitans in an Immuno-competent Young Girl
Empyema Necessitans (EN) is a variant of empyema that extends to extrapleural space and can affect chest wall too. Although, tuberculosis is the most common cause of EN, primary multidrug resistant TB presenting as EN is...
Unstable Distal Radius Fractures Treated by Volar Locking Anatomical Plates
Introduction: Fracture of the distal end of radius represents the most common fracture of the upper extremity accounting for approximately 16-20% of all fractures. Plating is now emerging as the gold standard for managem...
Esthesioneuroblastoma Presenting with Bilateral Proptosis and Blindness in a Child: A Case Report
Esthesioneuroblastoma is a rare malignant tumour of the olfactory epithelium. The most common symptom is related to unilateral nasal obstruction. It rarely presents with bilateral proptosis and blindness. We report a 21-...