Evaluation of the Xpert® MTB/RIF Assay and Microscopy for Diagnosing Pulmonary Tuberculosis in Butembo, Democratic Republic of the Congo
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 3
Abstract
Objective: This study aimed to evaluate the efficacy of Xpert® MTB/RIF assay comparing to the acid-fast smear microscopy for diagnosing pulmonary tuberculosis cases in Butembo, Democratic Republic of the Congo. Methodology: This was a retrospective study analyzing pulmonary tuberculosis cases that were diagnosed using both the Xpert® MTB/RIF assay and microscopy. Secondary data were collected from monthly report from the Microbiology department at GHR Katwa, Butembo, for the time period of February 2015 to September 2017. Results: Three hundred and seventy-two specimens were collected from patients suspected of pulmonary tuberculosis. Of these, 98 (26.3%) were found to be positive for MTB by Xpert® MTB/RIF assay, out of which 73 (19.6%) were also found to be positive by microscopy. The remainder was negative. Two (2.04%) cases of resistance to rifampicin were detected. The sensitivity, specificity, positive predictive value and negative predictive value of Xpert assay were respectively 83.6%, 87.6%, 62.2% and 95.6%. Conclusion: The use of the Xpert® MTB/RIF coupled with microscopy as part of routine assay, permits rapid diagnosis of pulmonary MTB and detection of rifampicin resistance. It may help in the epidemiological control of pulmonary MTB.
Authors and Affiliations
Gabriel Kambale Bunduki, Adélard Kalima Nzanzu, Alexandre Amini Mitamo
Keywords
Related Articles
- EP ID EP338270
- DOI 10.9734/JAMB/2017/38667
- Views 77
- Downloads 0
Preservative Capacity of Some Commercial Cosmetics in Akure Metropolis, Nigeria
The preservative capacities of some commercial cosmetics obtained from Akure, Nigeria were investigated. The results showed that 90.90% of the cosmetic products were contaminated with microorganisms. Bacteria were found...
Aspergillus Xylanases
There has been tremendous growth and development in the use of xylanase in the biotechnology industry. The market trends reveal that xylanase takes major position of share up to 20% of the world enzymes market along with...
Prevalence of Listeria monocytogenes in Fresh and Raw Fish, Chicken and Beef
There has been recent outbreaks of food poisoning associated with Listeria monocytogenes. Most of the outbreaks were attributed to fresh meat and dairy products, and processed foods. This study was therefore carried out...
Effectiveness of Griess Nitrite Test on Screening Asymptomatic Bacteriuria in Pregnancy: A Cross Sectional Study in Harare, Zimbabwe
Aim: The aim of this study was to evaluate effectiveness of the Griess test on screening asymptomatic bacteriuria when compared to culture. Study Design: This study was conducted using the cross sectional study design. P...
Development of Antibiotic Resistance in Herbal Drug-sensitized Staphylococcus aureus Isolate
Background: Herbal remedies are locally produced drugs usually made from plant sources. Due to their inexpensive nature, low-income individuals prefer them over modern antibiotics in the treatment of infections. Aims: T...