Evaluation of tolerance to Finale ® in germination and regeneration in vitro of two Cuban varieties of rice (IACuba-17 and IACuba-19)
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
Selection agent used during the shoot selection has an important role on the transgenic plant generation efficiency. In this work, the tolerance to the herbicide Finale ® in two Cuban rice cultivars, IACuba17 and IACuba-19 was evaluated, and determined that 10 days exposure to 5 and 10 mg.l -1 of Finale ® were enough to avoid seedlings of IAC-17 and IAC-19, respectively. Cultivated calluses (0, 2, 4 and 6 days) in the absence of Finale ® in the regeneration medium were used to evaluate the minimal concentration of Finale ® that totally inhibits shoot regeneration. Pre-induced calluses cultured during two days and 3 mg.l -1 of Finale ® in the regeneration medium was the most efficient combination to select shoots during the generation of transgenic plants resistant to the herbicide. Our shoot selection procedure reduces to 3 weeks the time to obtain shoots during the generation of transgenic rice plants.
Authors and Affiliations
Daymí Abreu , Maylin Pérez, Annerys González, Julio Alfonso, Onel Valdivia, Carlos Hernández, Raúl Armas
Keywords
Related Articles
- EP ID EP684217
- DOI -
- Views 141
- Downloads 0
In vitro multiplication of Macleania rupestris (Kunth) A.C. Sm. (Ericaceae)
Macleania rupestris (Kunth) A.C. Smith (Ericaceae) is a plant native to the Andes, which produces edible fruits with great food and commercial potential. M. rupestris plants have been in vitro established from seeds of...
Effect of filtered bacterial cultures with antifungal activity in vitro in the interaction Musa spp.- Mycosphaerella fijiensis
Although certain bacterial species may be in vitro antagonistic of Mycosphaerella fijiensis, as it was demonstrated, they are discrete results that have their effect on the Musa spp.-M. fijiensis interaction. The resul...
In vitro multiplication of Morinda royoc L. in Temporary Immersion Systems
Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of...
Acclimatization of plants of Carica papaya var. Red maradol obtained by somatic embryogenesis
Acclimatization stage of in vitro cultured papaya (Carica papaya L.) remains one of the main problems. This study evaluated the influence of the type of cover and length of in vitro plants for their survival in the accl...
Somatic embryogenesis in Carica papaya L. var. Red Maradol
The most important papaya variety in Cuba is Red Maradol. However, it is susceptible to the main viral diseases that attack this crop. For this reason, it is necessary to establish a method for plant regeneration via som...