Evaluation of tolerance to Finale ® in germination and regeneration in vitro of two Cuban varieties of rice (IACuba-17 and IACuba-19)
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
Selection agent used during the shoot selection has an important role on the transgenic plant generation efficiency. In this work, the tolerance to the herbicide Finale ® in two Cuban rice cultivars, IACuba17 and IACuba-19 was evaluated, and determined that 10 days exposure to 5 and 10 mg.l -1 of Finale ® were enough to avoid seedlings of IAC-17 and IAC-19, respectively. Cultivated calluses (0, 2, 4 and 6 days) in the absence of Finale ® in the regeneration medium were used to evaluate the minimal concentration of Finale ® that totally inhibits shoot regeneration. Pre-induced calluses cultured during two days and 3 mg.l -1 of Finale ® in the regeneration medium was the most efficient combination to select shoots during the generation of transgenic plants resistant to the herbicide. Our shoot selection procedure reduces to 3 weeks the time to obtain shoots during the generation of transgenic rice plants.
Authors and Affiliations
Daymí Abreu , Maylin Pérez, Annerys González, Julio Alfonso, Onel Valdivia, Carlos Hernández, Raúl Armas
Keywords
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