Evaluation the bovine oviduct epithelial cells culture in monolayer system
Journal Title: Scientific Journal of Veterinary Advances - Year 2014, Vol 3, Issue 4
Abstract
The oviduct is an important site in reproduction, such as fertilization and early embryonic development. In this study bovine oviduct epithelial cells (BOEC) isolated and cultured in monolayer system to evaluate the proliferation and viability of cells in quality manner before and after two time passages. The epithelial cells isolated mechanically from oviduct tissue in sterile condition. Isolated cells floated in culture medium, fetal bovine serum and antibiotics in a T-24 culture flask. Two days after seeding have been observed that cells adhered to flask surface and are proliferating. The rate of cell proliferation was well. After one week the cells covered the flask surface and were confluent. Then the cells isolated from flask by trypsin-EDTA enzyme and passage into 4 flasks. Cells cover the flasks in 10 days and passage into 12 flasks. In the second passage the cells was confluent in 12 days. We observed that in first culture there was a little dead cell during the culture and cells proliferation rate was high. After each passage the number of dead cells in flasks increased and cells proliferation rate in each passage decreased.
Authors and Affiliations
S. Erfani*| department of animal science, Faculty of Agriculture. University of Zanjan, Zanjan, IRAN., M. T. Harkinezhad| research institute of Biotechnology, University of Zanjan, Zanjan, IRAN., H. Amanlou| department of animal science, Faculty of Agriculture. University of Zanjan, Zanjan, IRAN.
Keywords
Related Articles
- EP ID EP914
- DOI 10.14196/sjvs.v3i4.1294
- Views 433
- Downloads 22
Participatory surveillance of livestock and poultry diseases in Agidi development area of Nasarawa state Nigeria
A participatory surveillance of livestock and poultry diseases was carried out in Agidi Development Area of Nasarawa State among 123 farmers, 29 of the respondent were female, while 94 were male. Open-ended interviews we...
Comparison of three suture techniques in closure of caprine skin incision: cosmetic and cost considerations
The cosmetic and economic evaluations of three suture techniques were evaluated in closure of caprine skin incision. Fifteen apparently healthy male and female intact goats, free of any dermatological lesions were used f...
Effect of different energy sources on feed consumption, weight gain and feed conversion ratio in broilers
The 400 straight run, day old Vencobb broilers chicks were randomly divided into four treatment groups of 100 birds each viz., control, T1, T2 and T3. Each group was further divided into two replications of 50 birds each...
Hematological and carcass characteristics of crossbred local and large white pigs fed with graded levels of chicken and fish viscera as feed supplement
The study aimed to evaluate the effect of chicken and fish viscera used as feed supplement in pigs on fattening. Ninety crossbreed (Local x Large White) weaned pigs of 12 weeks old, weighing on average 9.5kg were divided...
Prevalence of mastitis in goat herds in some northwestern villages in Nigeria
In this study 10 different herds were randomly sampled and milk samples obtained from 263 lactating does with normal sized udder and enlarged (unilateral/bilateral) pendulous udders .The obtained milk samples were analyz...