EVOLUTION OF NANOTECH ASSISTED PCR DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS AND ITS ASSESSMENT WITH CONVENTIONAL METHODS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 3
Abstract
Objective: This study was focused on assessment and performance of conventional and nanotech assisted methods for detection of Mycobacterium tuberculosis. Tuberculosis (TB) still leftovers one of the top ten causes of death worldwide, thus this study has undertaken the use of MNP for early detection of TB.Methods: A cross-sectional studies were conducted on clinical and radiological suspected TB patients in the department of microbiology at D. Y. Patil hospital Kolhapur. All samples received in the month of August 2016 to January 2017. Total One hundred twenty-sputum samples were processed for diagnosis of TB by ZN stain, culture on L. J. Medium and real-timePCR tests.Results: In our present study, 60.83% (73) patient male and 39.17 % (47) were female patient, showing a borderline male prevalence found in our study. Eighteen percent patients were found to be in the age group (21-30 y) are mostly affected for pulmonary tuberculosis. A significant difference was seen in the percentage of differentDNA extraction methods, the fig. being conventional chloroform-phenol 66.66%, the commercial kit 80%, magnetic bead 86.66% and MNPs method 99.66% found to be significant (P<0.0001**).Conclusion: In the present study the MNP-DNA extraction techniques with NALC followed by IS6110 target amplification were found superior for diagnosis of TB. The MNP assisted extraction method showed better results in terms of quantification and sensitivity of TB PCR diagnosis, evolving nanotech assisted innovative method.
Authors and Affiliations
Deepak V. Sawant, M. M. Desai, R. S. Patil, S. H. Pawar
Keywords
Related Articles
- EP ID EP567560
- DOI 10.22159/ijpps.2018v10i3.24085
- Views 83
- Downloads 0
COST-EFFECTIVENESS OF BETA BLOCKERS AND THE SURVIVAL OF THE PATIENTS WITH HEART FAILURE IN THE CARDIOLOGY CENTER IN TIRANA
Objective: To evaluate the survival of the patients with heart failure in the Cardiology Center in Tirana, to evaluate the cost effectiveness of carvedilol versus metoprolol.Methods: 239 patients (pts) suffering chronic...
ASSESSMENT EFFECT OF ALOE VERA, AZADIRACHTA INDICA AND MORINGA OLEIFERA AQUEOUS EXTRACTS ON CARBON TETRACHLORIDE-INDUCED HEPATOTOXICITY IN RATS
Objective: This experiment aims to investigate the hepatotherapeutic effect of Aloe vera (AV), Azadirachta indica (N), and Moringa oleifera (MO).Methods: Eighty albino rats have been divided into ten groups. The first gr...
LEVEL OF ADHERENCE TO LIFESTYLE CHANGES AND MEDICATIONS AMONG MALE HYPERTENSIVE PATIENTS IN TWO HOSPITALS IN TAIF; KINGDOM OF SAUDI ARABIA
Objective: The main aims were to measure adherence to diet, exercise and medications among hypertensive patients and to identify determinants of adherence if any.Methods: A cross-sectional study was conducted during Octo...
A METHOD FOR DETERMINING 1,4-BENZOTHIAZINE DERIVATIVES IN RAT PLASMA BY HPLC AND ITS APPLICATION TO A PHARMACOKINETIC STUDY
Objective: The objective of the study was to develop, optimize and validate of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the determining 1,4-benzothiazine derivatives (AR13 and AR15)...
EVALUATION OF THE SUSPENDING PROPERTIES OF SHEA TREE GUM
Objective: Shea gum is found in large quantities in the northern part of Ghana. Its use in the pharmaceutical industry has been limited by lack of research into the possible uses of the gum as a pharmaceutical excipient....