EX VIVO STUDY OF GARCINIA MANGOSTANA L. (MANGOSTEEN) PEEL EXTRACT AND XANTHONES AS ANTI-ADIPOGENESIS IN HEPG2 CELL MODEL
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2015, Vol 4, Issue 3
Abstract
Background: Anti-adipogenesis is one of proposed mechanism for anti-obesity. Adipogenesis regulation of obesity is important, so identification of anti-adipogenic activity is a potential strategy to find anti-obesity agent. Aim: The aim of this study is to evaluate the anti-adipogenesis potential of Garcinia mangostana L. peel extract (GMPE) compared to xanthones in HepG2 cells line as model. Material and Methods: GMPE was performed based on maceration method using distilated ethanol 70% as the solvent. The level of triglyceride and cholesterol and the inhibitory activity of triglyceride (TG) and cholesterol (CHOL) in HepG2 cells were assayed and determined as the anti-adipogenesis parameter. Results: The most active subtance to lower the triglyceride level was showed by GMPE in every concentration followed by the garcinone-C, γ-mangostin, garcinone-D and α- mangostin respectivelly. The highest activity to decrease the cholesterol level was showed by GMPE and followed by γ-mangostin, α-mangostin, garcinone-c, garcinone-d respectively. Conclusion: GMPE posses the anti-adipogenesis potential in inhibiting TG and CHOL synthesis was better than any other xanthone (α- mangostin, γ-mangostin, garcinone-C and garcinone-D).
Authors and Affiliations
Lusiana Darsono| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Meilinah Hidayat| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Maesaroh Maesaroh| Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II. No. 9 Bandung 40163, West Java, Indonesia, Nurul Fauziah| Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II. No. 9 Bandung 40163, West Java, Indonesia, Wahyu Widowati| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Corresponding author email: wahyu_w60@yahoo.com
Keywords
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- EP ID EP11443
- DOI 10.5958/2319-5886.2015.00109.5
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