EX VIVO STUDY OF GARCINIA MANGOSTANA L. (MANGOSTEEN) PEEL EXTRACT AND XANTHONES AS ANTI-ADIPOGENESIS IN HEPG2 CELL MODEL
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2015, Vol 4, Issue 3
Abstract
Background: Anti-adipogenesis is one of proposed mechanism for anti-obesity. Adipogenesis regulation of obesity is important, so identification of anti-adipogenic activity is a potential strategy to find anti-obesity agent. Aim: The aim of this study is to evaluate the anti-adipogenesis potential of Garcinia mangostana L. peel extract (GMPE) compared to xanthones in HepG2 cells line as model. Material and Methods: GMPE was performed based on maceration method using distilated ethanol 70% as the solvent. The level of triglyceride and cholesterol and the inhibitory activity of triglyceride (TG) and cholesterol (CHOL) in HepG2 cells were assayed and determined as the anti-adipogenesis parameter. Results: The most active subtance to lower the triglyceride level was showed by GMPE in every concentration followed by the garcinone-C, γ-mangostin, garcinone-D and α- mangostin respectivelly. The highest activity to decrease the cholesterol level was showed by GMPE and followed by γ-mangostin, α-mangostin, garcinone-c, garcinone-d respectively. Conclusion: GMPE posses the anti-adipogenesis potential in inhibiting TG and CHOL synthesis was better than any other xanthone (α- mangostin, γ-mangostin, garcinone-C and garcinone-D).
Authors and Affiliations
Lusiana Darsono| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Meilinah Hidayat| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Maesaroh Maesaroh| Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II. No. 9 Bandung 40163, West Java, Indonesia, Nurul Fauziah| Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II. No. 9 Bandung 40163, West Java, Indonesia, Wahyu Widowati| Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, West Java, Indonesia, Corresponding author email: wahyu_w60@yahoo.com
Keywords
Related Articles
- EP ID EP11443
- DOI 10.5958/2319-5886.2015.00109.5
- Views 275
- Downloads 12
Osteopathic Manipulative Treatment of Primary Dysmenorrhea and Related Factors: A Randomized Controlled Trial
Objectives: This study is aimed to evaluate if the osteopathic manipulative treatment (OMT) is effective in patients with primary dysmenorrhea (PD). Methods: Randomized single-blinded controlled trial with OMT group and...
A STUDY OF MORPHOLOGY OF VERMIFROM APPENDIX IN 200 CASES
Aims: To study the various positions of vermiform appendix, and its relation to various diseases of the vermiform appendix, and average length and external diameter of the vermiform appendix. Materials & Methods: This...
Prevalence of β-lactamases in clinical isolates of Enterobacter cloacae in the West Bank-Palestine
The increasing spread of β-lactamase-producing pathogens represents an emerging serious public health threat specially to treat nosocomial infections. This study was carried out to determine the prevalence and molecula...
Effect of Continuous Care model on lifestyle modification in patients with hypertension: Randomized clinical trial study
Hypertension is the most important modifiable cardiovascular disease. One of the main factors affecting the risk of hypertension is people’s lifestyle, for which appropriate changes in lifestyle-related factors creati...
Effect of the peer supportive program on blood pressure changes in patients affected with hypertension: A randomized controlled trial
Hypertension is the biggest risk factor of death worldwide. The peer supportive program is one of low-cost programs which can be used to enhance patient information in chronic diseases. The aim of this study is to dete...