Examination of Giardia intestinalis with Direct Microscopy and Direct Fluorescent Antibody in Patients with Diarrhea
Journal Title: Turkish Journal of Parasitology - Year 2020, Vol 44, Issue 4
Abstract
Objective: In this study, our objective was to compare direct microscopic examination and direct fluorescence antibody (DFA) method for Giardia diagnosis in stool samples and to evaluate the possible risk factors related to Giardia infections. Methods: Stool samples of 185 patients with diarrhoea collected between June 2019 and July 2019 in Erzurum Yakutiye Research Hospital were included in the study. Microscopic examination of the samples was performed with native-lugol, and they were subsequently scanned by the indirect fluorescent assay microscope using the DFA method at 100-200X magnification. In addition, all patients filled a questionnaire prepared to determine the possible risk factors related to Giardia infection. Results: The age of the 185 participating patients who belonged to different groups was between 0 and 94 years. Giardia spp. cysts were detected in five stool samples (2.7%) using direct microscopic examination. Nine samples (4.9%) were DFA-positive. The incidence of giardiasis was noted to be 7.5% in children, 3.8% in adults, 7.3% in people living in rural areas, 2.9% in people living in urban areas, 10% in people having pets and 4.2% in people who do not have pets. Conclusion: By taking the DFA method as a reference, the sensitivity and specificity of the microscopic examination were found to be 44.4% and 99.4%, respectively. The Giardia positivity rate was higher in children, those living in rural areas, those having pets and those using well water as drinking water.
Authors and Affiliations
Ahmet Yılmaz, Hakan Uslu
Keywords
Related Articles
- EP ID EP693286
- DOI 10.4274/tpd.galenos.2020.6876
- Views 85
- Downloads 0
Endoparasites Determined by Fecal Examination in Sheep in Erzurum Province
Objective: The aim of the current study was to determine the presence and prevalence of Eimeria and helminth species in sheep raised in Erzurum province by using fecal examination. Methods: Faecal samples were collecte...
‘‘Anti-lice Protector Shampoo’’: Clinical Study Shows Lack of Efficacy of Coconut Oil Derivatives in the Elimination of Head Louse Infestation
Objective: Coconut oil and its derivatives are widely thought to kill head lice by occlusion and asphyxiation. There is no evidence in support of coconut derivative-based products sold to treat pediculosis. This study wa...
The Prevalence of Parasitic Contamination of Fresh Vegetables in Tehran, Iran
Objective: Parasitic diseases have created numerous health and economic problems in developing and developed countries. One of the most prevalent ways of transmitting diseases is by consuming raw vegetables that are cont...
A Case of Linognathus setosus (von Olfers, 1816) (Phthiraptera: Anoplura) Infestation in a Dog
This case report was prepared to give information about Linognathus setosus (von Olfers, 1816) detected on a 2-year-old male Rottweiler breed dog which was brought to a private veterinary clinic due to restlessness and i...
Ophthalmomyiasis Externa: A Case Report
Ophthalmomyiasis is the infestation of the eye with fly larvae. Oestrus ovis is one of the most common causes of ophthalmomyiasis worldwide. Herein, we describe a case of external ophthalmomyiasis caused by Oestrus ovis...