Examination of The Immobilization and Kinetics of The Laccase Enzyme on Various Clay Minerals
Journal Title: MAS Journal of Applied Sciences - Year 2023, Vol 8, Issue 2
Abstract
In this study, the immobilization of the laccase enzyme, which has a wide application area in the industry, was examined. For this purpose, commercially obtained laccase enzyme was immobilized on various clays using the physical adsorption method. The effects of pH, temperature, substrate concentration, and storage time on the activity of free and immobilized laccase were examined. As a result of the studies, the optimum pH and temperature for free laccase were obtained as 5.5 and 40 °C respectively and the optimum pH and temperature for all immobilized enzymes (bentonite, diatomite, and Bardakçı) were 5.5-6.0 and 40 °C respectively. The effects of pH and temperature on the activity of the immobilized laccase showed that the properties of the immobilized enzyme were the same as those of the free enzyme. The resulting kinetic constant values turned out to be quite close to each other. In addition, it was shown that adsorption did not significantly affect the kinetic properties of the enzyme. Only 20%-30% of immobilized laccase activity disappeared in 2 months. KM values for free enzyme and immobilized enzymes were found as 0.0700 mM, 0.0724 mM, 0.0831 mM, and 0.0935 mM and Vmax values were 0.0695, 0.0216, 0.0236, and 0.0233 mM-1, respectively. The KM value of the immobilized enzyme is greater than that of the free enzyme and the Vmax value is smaller. The increase in resistance of the immobilized enzyme to temperature change and storage time indicates that laccase immobilization on clay is beneficial for enzyme immobilization.
Authors and Affiliations
Fulya ÖZDEMİR, Zeki YALÇINKAYA
Keywords
Related Articles
- EP ID EP723687
- DOI 10.5281/zenodo.7956783
- Views 38
- Downloads 0
The Effects of Plant-Based Feed Additives on Productive and Reproductive Performans in Poultry
The poultry sector is very important in order to meet the nutritional needs of the increasing world population. Compared to other farm animals, poultry has a shorter production period and is a relatively inexpensive sour...
Investigation of the Activities of the Irrigation Associations in Samsun
Irrigation associations are farmer organizations that carry out irrigation management with their own organizations and play a role in the development of the agricultural sector and the protection of the country's water r...
Health Evaluation of Lambs and Goat Kids Raised in Bingöl
This investigation was conducted as a direct survey that evaluated the overall health of lambs and goat kids, as well as their mothers, on small farms that were raised in Bingöl. It had a total of 66 questions on 200 bus...
Factors Affecting the Migration from Rural to Urban Areas: A Case Study in Gürün District of Sivas province
Throughout history, people have migrated to other places by leaving their original places of residence for different reasons. Migration from rural areas to cities is also a common phenomenon, especially in developing cou...
Analysis of International Lighting Criteria and Lıghtıng Design in Light-Sensitive Areas
This study examines the amount of light and the quality of the lighting solution, the preservation of works of art, and the perception of museum environments. Illumination of works of art should not be considered as mere...