EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
Journal Title: Сільськогосподарська мікробіологія - Year 2015, Vol 21, Issue
Abstract
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process. Introduction ammonium form was less efficient. It was shown thet the intensity of nitrogen source use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum the optimal concentration of potassium nitrate was 2,5 g/l resulting in 10*10 cells/ml, while under the use of vegetative cells the optimal concentration of potassium nitrate was 1,0 g/l leading to the accumulation of 10*9 cells/ml. Use of spore inoculum had ensured the maximum productivity of extracellular polysaccharide synthesis. With the higher number of B. mucilaginosus subcultures in a vegetative state the efficiency of the exopolysaccharide synthesis decreased: after the second passage - by 20,0%, after the fourth - by 56,5%, after the sixth - at 127,8%. After eight passage the culture loses its ability to synthesize polysaccharide, especially on the medium with the N/C ratio 1:3 and resume this ability only after sporulation and the significant increase of the N/C ratio.
Authors and Affiliations
I Malynovska
Keywords
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