EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
Journal Title: Сільськогосподарська мікробіологія - Year 2015, Vol 21, Issue
Abstract
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process. Introduction ammonium form was less efficient. It was shown thet the intensity of nitrogen source use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum the optimal concentration of potassium nitrate was 2,5 g/l resulting in 10*10 cells/ml, while under the use of vegetative cells the optimal concentration of potassium nitrate was 1,0 g/l leading to the accumulation of 10*9 cells/ml. Use of spore inoculum had ensured the maximum productivity of extracellular polysaccharide synthesis. With the higher number of B. mucilaginosus subcultures in a vegetative state the efficiency of the exopolysaccharide synthesis decreased: after the second passage - by 20,0%, after the fourth - by 56,5%, after the sixth - at 127,8%. After eight passage the culture loses its ability to synthesize polysaccharide, especially on the medium with the N/C ratio 1:3 and resume this ability only after sporulation and the significant increase of the N/C ratio.
Authors and Affiliations
I Malynovska
Keywords
Related Articles
- EP ID EP520080
- DOI -
- Views 102
- Downloads 0
cORN ROOT AREA MYCOCENOSIS UNDER THE INFLUENCE OF CHAETOMIUM GLOBOSUM AS DESTRUCTOR OF STRAW
Quantitative and qualitative composition of fungi in the rhizosphere soil of corn was studied. It was shown that introduction of straw into the soil resulted in an increase the number of representatives of Fusarium Link...
INFLUENCE OF ULTRAVIOLET RADIATION ON THE VIABILITY OF VEGETATIVE CELLS AND CYSTS OF AZOTOBACTER CHROOCOCUM 2.1
Objective. Study the influence of ultraviolet radiation (UV radiation) on the preservation of cysts and vegetative cells of Azotobacter chroococcum 2.1 in the digest medium and seeds of cucumber, including for the use of...
BRADYRHIZOBIUM JAPONICUM REACTION IN PURE CULTURE AND SYMBIOTIC SYSTEMS TO THE USE OF NANOCARBOXYLATES OF MICROELEMENTS
Objective. Study the influence of various concentrations of germanium, molybdenum, vanadium, cobalt, iron, copper and zinc on the growth dynamics of rhizobia, to select the most effective ones for studying their role as...
EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process....
THE CORRECTIVE EFFECT OF CAROTENEPRODUCED YEAST PHAFFIA RHODOZYMA BIOMASS ON INTESTINAL MICROBIOCENOSIS FORMATION OF DUCKS
The changes the of intestinal microflora composition of Beijing broiler ducks at intro¬duction of caroteneproduced yeast Phaffia rhodozyma biomass to the diet was studied. The positive changes of 37-days ducks intestinal...