EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM
Journal Title: Сільськогосподарська мікробіологія - Year 2015, Vol 21, Issue
Abstract
It was established that B. mucilaginosus С-3 requires introduction of nitrogen source into the culture medium in form of nitrate as the most optimal for maximal accumulationof bacterial biomass and sporulation process. Introduction ammonium form was less efficient. It was shown thet the intensity of nitrogen source use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum the optimal concentration of potassium nitrate was 2,5 g/l resulting in 10*10 cells/ml, while under the use of vegetative cells the optimal concentration of potassium nitrate was 1,0 g/l leading to the accumulation of 10*9 cells/ml. Use of spore inoculum had ensured the maximum productivity of extracellular polysaccharide synthesis. With the higher number of B. mucilaginosus subcultures in a vegetative state the efficiency of the exopolysaccharide synthesis decreased: after the second passage - by 20,0%, after the fourth - by 56,5%, after the sixth - at 127,8%. After eight passage the culture loses its ability to synthesize polysaccharide, especially on the medium with the N/C ratio 1:3 and resume this ability only after sporulation and the significant increase of the N/C ratio.
Authors and Affiliations
I Malynovska
Keywords
Related Articles
- EP ID EP520080
- DOI -
- Views 80
- Downloads 0
PECULIARITIES OF USE OF MODERN BIOLOGICAL PREPARATIONS FOR PROTECTION OF AGRICULTURAL CULTURE FROM PESTS IN THE FOREST STEPPE AND POLISSIA OF UKRAINE
The modern biological preparation and their efficiency in the system of protection of agricultural crops from pests, in particular trichogramma, natural populations of the common lacewing, as well as biopreparations Bito...
THE IMPACT OF BIOLOGICAL PREPARATIONS ON THE EFFECTIVE SOIL FERTILITY AND MINERAL NUTRITION OF APRICOT AND CHERRY PLUM SEEDLINGS
The effect of microbial preparations (MP) use, created on the basis of nitrogen-fixing and phosphorous mobilising bacteria as well as complex of microbial preparations (CMP) on the effective soil fertility, mineral nutri...
TREND OF PROCESSES OF BIOLOGICAL TRANSFORMATION OF NITROGEN IN RHIZOSPHERIC SOIL OF POTATO PLANTS UNDER BIOTIC AND ABIOTIC FACTORS OF CULTURE FERTILIZATION
In the stationary field experiment on sod-podzolic soil in terms of the parameters of trend of processes of nitrogen fixation and biological denitrification, environmentally appropriate levels and types of fertilization...
MICROBIAL PREPARATIONS IN AGRICULTURE CULTURAL TECHNOLOGIES AS A REGULATING FACTOR FOR THE ACTIVITY OF THE DENITRIFICATION PROCESS
Objective. To determine the features of potential N2O emission from the rhizospheric soil of plants under different crop fertilization systems. Methods. Field experiment, gas chromatographic methods. Results. In the fiel...
DYNAMICS OF POPULATIONS OF MICROORGANISMS IN CANNED CRIMPED GRAIN MAIZE WHEN IMPACT STRAINS OF LACTIC ACID BACTERIA
The dynamics of the development of microorganisms, changes in the level of acidity and the ratio of the accumulated organic acids in canned by flattening the grain of corn was investigated under the influence of strains...