Expansion of plasmid mediated blaACT-2 among Pseudomonas aeruginosa associated with hospital infection and its transcriptional response under cephalosporin stress

Journal Title: Journal of Microbiology and Infectious Diseases - Year 2017, Vol 7, Issue 2

Abstract

Objectives: Organisms harboring multiple plasmid mediated β-lactamases are major concerns in nosocomial infections. Among these plasmid mediated β-lactamases, ACT (EBC family) is a clinically important enzyme capable of hydrolyzing broad spectrum cephalosporins. Therefore, the present study was undertaken to determine the prevalence of ACT determinant along with other co-existing β-lactamase genes in P. aeruginosa strains. Methods: A total of 176 Pseudomonas isolates were phenotypically screened for the presence of AmpC β-lactamase by M3DET Method followed by Molecular detection using PCR assay. Transcriptional evaluation of blaACT-2 gene was analyzed by RT-PCR and its transferability was performed by transformation and conjugation. Results: Present study demonstrates the presence of ACT-2 allele among 12 strains of P. aeruginosa. Co-existence of other β-lactamase genes were encountered among ACT-2 harboring strains which includes CTX-M (n=2), SHV (n=3), TEM (n=2), VEB (n=2), OXA-10 (n=1), CIT (n=2) and DHA (n=3). Fingerprinting by REP PCR revealed the isolates harboring ACT-2 to be distinct and these isolates showed high resistance to expanded-spectrum cephalosporins and even to carbapenem group of drugs. This ACT-2 allele was encoded in the plasmid (L/M, FIA, FIB Inc. Group) and conjugatively transferable. Transcriptional analysis revealed a significant increase in ACT-2 expression (483 fold) when induced by ceftriaxone at 4 µg/ml followed by ceftazidime at 8 µg/ml (31 fold) and cefotaxime 4 µg/ml (8 fold). Conclusion: In this study detection of ACT-2 plasmid mediated AmpC β-lactamase along with other β-lactamase genes in clinical isolates of P. aeruginosa represents a serious therapeutic challenge. Therefore, revision in antimicrobial policy is required for effective treatment of patients infected with pathogen expressing this mechanism. J Microbiol Infect Dis 2017; 7(2): 75-82

Authors and Affiliations

Birson Ingti, Deepjyoti Paul, Anand Prakash Maurya, Debajyoti Bora, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee

Keywords

Related Articles

Surviving Mardi Gras in an Intensive Care Unit

Penicilliosis is a life threatening fungal infection caused by Penicillium marneffei (PM). It has a high mortality rate if not picked up in time and treated appropriately. Making an early diagnosis is a key in improving...

The incidence of fungal keratitis in Zagazig University Hospitals, Egypt and the value of direct microscopy and PCR technique in rapid diagnosis

Objective: To determine the frequency and risk factors of fungal keratitis (FK) and the value of direct microscopy and PCR techniques of corneal smears as appropriate diagnostic methods. Methods: The keratitis cases in...

Hepatit C tedavisinde karşılaşılan telaprevir ilişkili DRESS sendromu

Kronik hepatit C tedavisinde telaprevir yüksek kalıcı viral yanıt ve kısa süreli tedavi özelliğiyle dikkat çekmektedir, ancak özellikle çeşitli cilt yan etkileri olmak üzere yeni yan etkilerle ilişkilidir. Biz telaprevir...

Lemierre sendromu ve sağ adneks absesi: Bir olgu sunumu ve literatür incelemesi

Lemierre sendromu çok nadir görülen ve tamamen tedavi edilebilen bir durumdur. Erken tanı ve uygun tedavi için çok erkenden şüphelenmek gerekmektedir. Burada boğaz ağrısı, ateş, boyun ağrısı, karın ağrısını takip eden bu...

Pseudomonas aeruginosa from the Hospital Environment

Hospital acquired infection is an additional affliction to the patient admitted to the hospital for some serious illness and is caused by pathogens which are prevalent in hospital environment. In the hos-pital, microbes...

Download PDF file
  • EP ID EP413369
  • DOI 10.5799/ jmid.vi.328788
  • Views 88
  • Downloads 0

How To Cite

Birson Ingti, Deepjyoti Paul, Anand Prakash Maurya, Debajyoti Bora, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee (2017). Expansion of plasmid mediated blaACT-2 among Pseudomonas aeruginosa associated with hospital infection and its transcriptional response under cephalosporin stress. Journal of Microbiology and Infectious Diseases, 7(2), 75-82. https://europub.co.uk/articles/-A-413369