Experimental study of efficacy of intralamellar keratoplasty with corneal stromal substitute developed from decellularized porcine cornea

Journal Title: Офтальмологический журнал - Year 2017, Vol 0, Issue 3

Abstract

Backrgound: Enhancing methods for decellularization of animal corneas for the development of bioengineered corneal models with the following keratoplasty makes it possible to overcome the shortage of human donor material. Purpose: To investigate the clinical efficacy of intralamellar keratoplasty (IKP) with corneal stromal substitutes (CSSs) developed using different decellularization methods in the animal study. Materials and Methods: Corneas were excised from enucleated pig eyes, and, to develop acellular CSSs, five methods of decelluarization of porcine corneas were used that were different in the time and conditions of incubation with detergents (0.5% sodium dodecyl sulphate or TRITON X-100) and proteolytic enzymes (like 0.1% papain). In addition, an ultrasonic apparatus of type СD 3800 А was used to further improve the removal of cells and acellular corneal debris. A total of 40 Chinchilla rabbits were used in the animal study. They were divided into 5 groups with different CSS versions. In each rabbit, the monocular IKP was carried out with one of the five CSS versions. Postoperatively, antimicrobial and anti-inflammatory therapy was instituted. Animals were examined every other day for 30 days postoperative. This included corneal fluorescein staining. In addition, the following was assessed ophthalmoscopically: conjunctival discharge, conjunctival hyperemia, state of the CSS, state of the host cornea, and, at late follow-up examinations, graft acceptance and whether graft-versus-host disease was present. Results: In rabbit eyes that underwent IKP with a version 4 CSS, the clinical scores for conjunctival discharge, corneal graft edema, inflammatory infiltration, corneal graft opacity and corneal fluorescein staining (0.13 points) were statistically significantly lower than in eyes that underwent IKP with other CSS versions. Location of inflammation in the cornea was found to be paracentral in eyes that improved clinically after IKP with version 1, 2, 3 or 5 CSS, and tended to be central (0.38 points) in those that underwent IKP with a version 4 CSS. Conclusion: The least apparent reaction was noted in the rabbit eyes that underwent IKP with a version 4 CSS, which makes this CSS promising for further investigations in pre-clinical and clinical studies.

Authors and Affiliations

N. Pasyechnikova, B. Cogan, S. Kolomiichuk

Keywords

Related Articles

Character of meibomian gland dysfunction in patients with diabetic polyneuropathy with severe complications

Background. Meibomian gland dysfunction (MGD) is a chronic, diffuse abnormality of the meibomian glands, commonly characterized by terminal duct obstruction and/or qualitative/quantitative changes in the glandular secret...

On the scientific results of the 3rd Global Pediatric Ophthalmology Congress (London 2018) and on the effectiveness of publishing articles in high-ranked ophthalmology journals

In this day and age of information technology and significantly accelerated scientific and technical progress in all fields of knowledge including ophthalmology, it is crucial for scientific results obtained to be publis...

Spectroscopic analysis of intraocular fluid in patients with cataract and pseudoexfoliation syndrome-associated glaucoma

Background: Pseudoexfoliation syndrome (PES) is a risk factor for the development of primary open angle glaucoma (POAG). Impaired intraocular fluid (as reflected by the chemical composition and physical properties, veloc...

Status of humoral immune factors in peripheral blood of fronto-orbital trauma patients late after surgery with the use of biocomposite

Purpose: To identify the influence of biocomposite on immune system status in front bone trauma (FBT) patients at 9 months or later after surgery involving the use of the composite material. Material and Methods: Twenty...

Clinical assessment of retinal ganglion cell complex changes in patient groups differing in severity of diabetic polyneuropathy

Background: To our knowledge, no clinical study has previously been performed to investigate changes in the retinal ganglion cell complex (GCC) in diabetes mellitus (DM) patients differing in severity of diabetic polyneu...

Download PDF file
  • EP ID EP249200
  • DOI -
  • Views 98
  • Downloads 0

How To Cite

N. Pasyechnikova, B. Cogan, S. Kolomiichuk (2017). Experimental study of efficacy of intralamellar keratoplasty with corneal stromal substitute developed from decellularized porcine cornea. Офтальмологический журнал, 0(3), 48-55. https://europub.co.uk/articles/-A-249200