Exploration of the antithrombotic effect of a C type lectin purified from Cerastes cerastes venom by protein-protein docking

Journal Title: UNKNOWN - Year 2018, Vol 2, Issue 2

Abstract

Background: Thromboembolic diseases are a major clinical problem due to their high prevalence and their often fatal consequences. In the present study, an anticoagulant galactoside binding C type lectin “Cc-Lec” was purified and characterized. Methods: Cc-Lec was purified by affinity chromatography on a column of Sepharose 4B coupled with D-lactose. Its homogeneity was verified by SDS-PAGE and ESI-MS. Cc-Lec 3D structure modelization was achieved by homology to Convulxin a snake venom C type lectin. Cc-Lec anticoagulant effect was explored in vivo by i.p. administration to mice, in vitro by native PAGE analysis and in silico by protein-protein docking approach. Results: Cc-Lec is a 34 271,59 Da protein, composed of 160 residues of amino acids for each subunit. Its 3D structure is organized into a homodimer cross-linked with a disulfide bridge and composed of three alpha helices and seven beta strands for each monomer. Cc-Lec functional characterization revealed a durable anticoagulant effect in vivo after 6 and 48h of i.p. administration to mice. This anticoagulant effect is mediated by interaction with FXa and FIXa as showed by native PAGE analysis. Moreover, protein-protein docking results reinforced this data and showed that Cc-Lec interacts with coagulation factors X and IX through their -carboxyglutamic domains. The interaction with factors X and IX requires calcium or calcium and magnesium ions respectively. Conclusion: The anticoagulant effect of Cc-Lec makes it a promising pharmacological target for the diagnosis and/or the therapy of the thromboembolic diseases.

Authors and Affiliations

Fatah Cherifi, Samah Saoud, Fatima Laraba-Djebari

Keywords

Related Articles

Detection of muscle fatigue, from statistical methods to software applications

Background: Muscle fatigue has become increasingly present in our daily lives. This is related to lifestyle difficulties. Several methods were proposed in order to detect the muscle fatigue. This report proposes a short...

Multimodality molecular imaging: Paving the way for personalized medicine

Early diagnosis and therapy increasingly operate at the cellular, molecular or even at the genetic level. As diagnostic techniques transition from the systems to the molecular level, the role of multimodality molecular i...

Reviews and Sensitization About Cancer in Algeria

Having cancer data is a necessity for several reasons. Researchers need accurate data and updates on cancer for the study of possible causes, managers for decisions concerning the acquisition of equipment and/or developm...

Explainer: An interactive Agent for Explaining the Diagnosis of Cardiac Arrhythmia Generated by IK-DCBRC

Interactions between medical applications and users involve a high level of trust, since many complex, automated applications are integrated and involve critical domains in which public health is paramount. Although unce...

Algorithmic processing to aid in leukemia detection

Background: I present our medical context with some basic concepts in order to understand the results of our work, and then I begin the explanation of mathematical morphology. I will conclude by the description of algori...

Download PDF file
  • EP ID EP648632
  • DOI 10.26415/2572-004X-vol2iss2p226-227
  • Views 80
  • Downloads 0

How To Cite

Fatah Cherifi, Samah Saoud, Fatima Laraba-Djebari (2018). Exploration of the antithrombotic effect of a C type lectin purified from Cerastes cerastes venom by protein-protein docking. UNKNOWN, 2(2), 226-227. https://europub.co.uk/articles/-A-648632