Exploring Antipruritic and Analgesic Effects of Matrine by Allergic Contact Dermatitis Mouse Model through Behavioral Analysis
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2017, Vol 7, Issue 4
Abstract
Aim This study investigated the antipruritic and analgesic effects of matrine on allergic contact dermatitis (ACD) through behavioral analyses and cheek fold thickness. To provide theoretical evidences for further development and application of antipruritic and analgesic traditional Chinese medicines (TCM), the contact sensitizer squaric acid dibutylester (SADBE) was used to elicit contact hypersensitivity in the mice as a model of ACD in humans. Methods C57BL/6 mice were divided into control group, model group, matrine groups (including 80 mg/kg, 40 mg/kg and 20 mg/kg dose groups), and dexamethasone acetate group (positive control). The sensitizer was first applied on the mice abdomen for sensitization and then on the cheek for challenge to elicit ACD. We confirmed the antipruritic and analgesic effects of matrine by comparing the spontaneous scratching and wiping directed to the cheek as well as the changes of cheekfold thickness in the different groups of mice. Results The scratching of model group was increased significantly when it compared with the control group (P < 0.05). Compared to the model group, the scratching of each matrine group decreased significantly (P < 0.05); The wiping of model group was increased significantly when it compared to control group (P < 0.01). Compared to the model group, the wiping of each matrine group decreased significantly (P < 0.01); The changes of skin fold thickness of model group increased significantly when it compared to the control group (P < 0.01). Compared to model group, the changes of the skin fold thickness of each matrine group decreased significantly (P < 0.01). Conclusion We have successfully established an ACD model that provides a behavioral differentiation by itch-like scratching and pain-like wiping in mice. Results proved that matrine can obviously improve the itch and pain sensations of ACD mice, and the effects of matrine has a dose-dependent manner obviously.
Authors and Affiliations
Xiaoyun Xu, Yixi Yan, Jianhao Pan, Zhe Zhang, Aishu Song, Tao Zhu, Hong Nie
Keywords
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