Exploring Antipruritic and Analgesic Effects of Matrine by Allergic Contact Dermatitis Mouse Model through Behavioral Analysis
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2017, Vol 7, Issue 4
Abstract
Aim This study investigated the antipruritic and analgesic effects of matrine on allergic contact dermatitis (ACD) through behavioral analyses and cheek fold thickness. To provide theoretical evidences for further development and application of antipruritic and analgesic traditional Chinese medicines (TCM), the contact sensitizer squaric acid dibutylester (SADBE) was used to elicit contact hypersensitivity in the mice as a model of ACD in humans. Methods C57BL/6 mice were divided into control group, model group, matrine groups (including 80 mg/kg, 40 mg/kg and 20 mg/kg dose groups), and dexamethasone acetate group (positive control). The sensitizer was first applied on the mice abdomen for sensitization and then on the cheek for challenge to elicit ACD. We confirmed the antipruritic and analgesic effects of matrine by comparing the spontaneous scratching and wiping directed to the cheek as well as the changes of cheekfold thickness in the different groups of mice. Results The scratching of model group was increased significantly when it compared with the control group (P < 0.05). Compared to the model group, the scratching of each matrine group decreased significantly (P < 0.05); The wiping of model group was increased significantly when it compared to control group (P < 0.01). Compared to the model group, the wiping of each matrine group decreased significantly (P < 0.01); The changes of skin fold thickness of model group increased significantly when it compared to the control group (P < 0.01). Compared to model group, the changes of the skin fold thickness of each matrine group decreased significantly (P < 0.01). Conclusion We have successfully established an ACD model that provides a behavioral differentiation by itch-like scratching and pain-like wiping in mice. Results proved that matrine can obviously improve the itch and pain sensations of ACD mice, and the effects of matrine has a dose-dependent manner obviously.
Authors and Affiliations
Xiaoyun Xu, Yixi Yan, Jianhao Pan, Zhe Zhang, Aishu Song, Tao Zhu, Hong Nie
Keywords
Related Articles
- EP ID EP433036
- DOI -
- Views 138
- Downloads 0
Cervical Lymphadenopathy and Its Management: An Interventional Study
Background Cervical lymphadenopathy refers to lymphadenopathy of the cervical lymph nodes (the glands in the neck), the term lymphadenopathy strictly speaking refers to disease of the lymph nodes. Objectives To diagnose...
The Revolution in Endodontic Imaging: Extraoral Periapical Radiography
Aim To evaluate the accuracy of extraoral periapical radiography in all phases of endodontic therapy and to determine the magnification error by comparing it with the radiographs obtained from the standard intraoral appr...
Paediatric Appendicitis: Three-year Experience in a Secondary Care Hospital
Objective To analyze cases of acute appendicitis in the pediatric population and find the effectiveness of ultrasonography and laparoscopic appendectomy in patients attending the referral hospital of Andaman & Nicobar Is...
Cesarean Versus Vaginal Delivery in Preterm Singletons – A Prospective Study
Aim To determine whether vaginal delivery or caesarean delivery is associated with a better neonatal outcome in preterm infants (30–36 weeks). Design All cases admitted to H.M.CH, Labour room between 2014 and 2016 were i...
A Study on Budesonide/ Formoterol vs. Fluticasone/ Salmeterol Inhaled Combination in Moderate to Severe Asthma
Background and Objectives The addition of an inhaled long-acting β2-agonist (LABA) to an inhaled corticosteroid (ICS) gives optimal control of asthma in most patients. The LABA salmeterol xinafoate (salmeterol) with inha...